Abstract: Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide.

Abstract: Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.

Abstract: Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.

Abstract: Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide.

Abstract: The invention is directed to a suspension structure that includes an internal height sensor assembly. The height sensor assembly monitors the height of the structure, i.e., the distance between first and second sides of the suspension structure. If the height changes, the height sensor assembly may provide this information to a control circuit so that adjustments to the spring constant of the suspension structure can be made. The height sensor assembly may include a reel assembly attached to a first side of the suspension structure, and a cord wound about the reel assembly and attached to a second side of the suspension structure. Detectable elements on the reel assembly or the cord may facilitate height measurements via a sensor circuit. Alternatively, the height sensor assembly may include a magnetic sensor that detects a position of a magnet which moves in response to winding or unwinding of the cord.