Patents by Inventor Amar Gupta

Amar Gupta has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS FOR PERFORMING MULTIPLEXED PCR
    Publication number: 20180073056
    Abstract: The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: September 15, 2017
    Publication date: March 15, 2018
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for the enrichment of mutated nucleic acid from a mixture
    Patent number: 9873908
    Abstract: The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: January 23, 2018
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Amar Gupta, Nancy Schoenbrunner, Kevin Janssen
  • Nucleic acid target identification by structure based probe cleavage
    Patent number: 9828629
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: November 28, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • MULTIPLEXED NUCLEIC ACID TARGET IDENTIFICATION BY STRUCUTRE BASED PROBE CLEAVAGE
    Publication number: 20170204462
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Application
    Filed: March 28, 2017
    Publication date: July 20, 2017
    Inventor: Amar Gupta
  • GENERIC METHOD FOR THE STABILIZATION OF SPECIFIC RNA
    Publication number: 20170183720
    Abstract: The present invention relates to methods and compositions for the stabilization of specific RNA molecules that can either be the target for detection or the control standard.
    Type: Application
    Filed: December 20, 2016
    Publication date: June 29, 2017
    Inventor: Amar Gupta
  • Multiplexed nucleic acid target identification by strucutre based probe cleavage
    Patent number: 9637791
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Grant
    Filed: December 8, 2014
    Date of Patent: May 2, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • Compounds and methods for the enrichment of mutated nucleic acid from a mixture
    Patent number: 9279146
    Abstract: The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes novel compounds and methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.
    Type: Grant
    Filed: December 6, 2013
    Date of Patent: March 8, 2016
    Assignee: ROCHE MOLECULAR SYSTEMS, INC.
    Inventors: Amar Gupta, Nancy Schoenbrunner
  • METHOD FOR VISUAL IDENTIFICATION OF PCR SOLUTIONS FOR ACCURATE REACTION SETUP
    Publication number: 20160053302
    Abstract: The present invention provides for methods and compositions that use visible dyes for the identification of reagents and solutions that are used to perform PCR assays.
    Type: Application
    Filed: April 16, 2015
    Publication date: February 25, 2016
    Inventors: Amar Gupta, Nancy Schoenbrunner, Kevin Janssen, Victoria Brophy
  • NUCLEIC ACID TARGET IDENTIFICATION BY STRUCUTRE BASED PROBE CLEAVAGE
    Publication number: 20150376681
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Application
    Filed: September 17, 2014
    Publication date: December 31, 2015
    Inventor: Amar Gupta
  • Sequencing and genotyping using reversibly terminating nucleotides
    Patent number: 9206476
    Abstract: The invention provides a method of determining the nucleotide sequence of a target nucleic acid using a reversibly terminating nucleotide that is modified at the 2? position.
    Type: Grant
    Filed: December 20, 2006
    Date of Patent: December 8, 2015
    Assignee: ROCHE MOLECULAR SYSTEMS, INC
    Inventors: David H. Gelfand, Amar Gupta
  • Method For Coding Of Multiple PCR Reactions For Assay Recognition
    Publication number: 20150176060
    Abstract: The present invention provides for methods and compositions that use fluorescent dyes for the identification of reagents and solutions that are used to perform PCR assays.
    Type: Application
    Filed: December 8, 2014
    Publication date: June 25, 2015
    Inventors: Amar Gupta, Nancy Schoenbrunner, Kevin Janssen, Nicolas Newton
  • MULTIPLEXED NUCLEIC ACID TARGET IDENTIFICATION BY STRUCUTRE BASED PROBE CLEAVAGE
    Publication number: 20150176075
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Application
    Filed: December 8, 2014
    Publication date: June 25, 2015
    Inventor: Amar Gupta
  • Methods for the Enrichment of Mutated Nucleic Acid from a Mixture
    Publication number: 20150147760
    Abstract: The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.
    Type: Application
    Filed: November 19, 2014
    Publication date: May 28, 2015
    Inventors: Amar Gupta, Nancy Schoenbrunner, Kevin Janssen
  • Compounds and Methods for the Enrichment of Mutated Nucleic Acid from a Mixture
    Publication number: 20140308664
    Abstract: The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes novel compounds and methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.
    Type: Application
    Filed: December 6, 2013
    Publication date: October 16, 2014
    Applicant: Roche Molecular Systems, Inc.
    Inventors: Amar Gupta, Nancy Schoenbrunner
  • NUCLEIC ACID TARGET IDENTIFICATION BY STRUCUTRE BASED PROBE CLEAVAGE
    Publication number: 20140272955
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Application
    Filed: March 10, 2014
    Publication date: September 18, 2014
    Applicant: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • Method for the prevention of carryover contamination in nucleic acid amplification technologies
    Patent number: 8669061
    Abstract: An improved method of preventing carryover contamination of an amplification reaction involves treating uracil-containing DNA with uracil-N-DNA glycosylase and heating the DNA in the presence of polyamines, such as spermidine, spermine and the like. Alternatively, after treatment with uracil-N-DNA glycosylase, the reaction is further incubated with an enzyme having AP lyase activity.
    Type: Grant
    Filed: June 16, 2009
    Date of Patent: March 11, 2014
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Amar Gupta, Stephen Gordon Will, Roy Bohenzky, Janine Montiel
  • 2?-terminator nucleotide-related methods and systems
    Patent number: 8163487
    Abstract: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2?-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: April 24, 2012
    Assignee: Roche Molecular Systems, Inc.
    Inventors: David Harrow Gelfand, Fred Lawrence Reichert, Veeraiah Bodepudi, Amar Gupta, Stephen Will, Thomas W. Myers
  • 2'-Terminator Nucleotide-Related Methods and Systems
    Publication number: 20110201002
    Abstract: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2?-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
    Type: Application
    Filed: March 10, 2011
    Publication date: August 18, 2011
    Applicant: ROCHE MOLECULAR SYSTEMS, INC.
    Inventors: David Harrow Gelfand, Fred Lawrence Reichert, Veeraiah Bodepudi, Amar Gupta, Stephen Will, Thomas Myers
  • 2?-Terminator nucleotide-related methods and systems
    Patent number: 7919249
    Abstract: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2?-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
    Type: Grant
    Filed: July 16, 2008
    Date of Patent: April 5, 2011
    Assignee: Roche Molecular Systems, Inc.
    Inventors: David Harrow Gelfand, Fred Lawrence Reichert, Veeraiah Bodepudi, Amar Gupta, Stephen Will, Thomas Myers
  • Compounds and methods for synthesis and purification of oligonucleotides
    Patent number: 7858772
    Abstract: The present invention provides methods of extending nucleic acids and purifying target nucleic acids. The methods include the use of capping reagents to effect chain termination and provide a handle for purification via fluorous affinity methods.
    Type: Grant
    Filed: December 18, 2007
    Date of Patent: December 28, 2010
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Amar Gupta, Stephen Will