Abstract: The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

Abstract: The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.

Abstract: The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.

Abstract: The present disclosure provides compositions and methods for controlling insect pests with bioactive agents. Also, the present disclosure provides insecticidal compositions and methods of using the formulations containing minicells and bioactive agents for targeted delivery and controlled release to enhance insect control in an environment-friendly, stable and scalable manner.

Abstract: The present disclosure provides compositions and methods for controlling fungal pests with bioactive agents. Also, the present disclosure provides fungicidal compositions and methods of using the formulations containing minicells and bioactive agents for targeted delivery and controlled release to enhance control of pathogenic fungi in an environment-friendly, stable and scalable manner.

Abstract: The present disclosure provides biodegradable, bioactive biopolymer nanocoating platforms, compositions thereof, and methods for making and producing the biopolymer nanocoating platforms. The present disclosure also provides various agricultural applications of the biodegradable, bioactive biopolymer nanocoating platforms.

Abstract: The present disclosure provides compositions and methods for suppressing growth of various plant species with bioactive agents. Also, the present disclosure provides agricultural compositions and methods of using the formulations containing minicells and bioactive agents for targeted delivery and controlled release to enhance control of various plant species in a stable and scalable manner.

Abstract: The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.

Abstract: The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells.

Abstract: The present disclosure provides controlled bioprocessing systems for enhanced minicell production with defined fermentation parameters and methods of said bioprocessing systems and methods for the production of minicells from said bioprocessing systems using a bioreactor or fermenter.

Abstract: The present disclosure relates to compositions of immobilized enzymes on the surface of achromosomal and/or anucleate cells and uses thereof. In particular, the present disclosure provides genetically engineered minicells with enzymes self-assembled on their surface. The immobilized enzymes on the surface of achromosomal and/or anucleate minicells, has agricultural, industrial, and environmental applications due to their improved stability durability and, reusability. Also, provided are methods for producing and purifying enzyme-immobilized minicells.

Abstract: A system accesses a first digital audio file that includes a plurality of spoken instructions. The system converts the first digital audio file to a first spectrogram image, applies a filter to determine whether an image quality of the first spectrogram image is below a predetermined image quality, and in response, generates a second spectrogram image from the first spectrogram image using a training model. The system converts the second spectrogram image to a second digital audio file and converts the second digital audio file into multiple vectors that each correspond to a particular spoken instruction. The system identifies related vectors and concatenates the related vectors together in order to create a plurality of concatenated vectors. The system generates, using the plurality of concatenated vectors, a third digital audio file that includes concatenated spoken instructions from the first digital audio file.

Abstract: It is herewith provided a biocontrol composition comprising an intact minicell comprising a nucleic acid that targets a transcript encoding a polypeptide and a method for controlling pests and pathogens with novel systems and compositions to deliver RNA molecules to a subject.

Abstract: The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

Abstract: The present disclosure relates to compositions of immobilized enzymes on the surface of achromosomal and/or anucleate cells and uses thereof. In particular, the present disclosure provides genetically engineered minicells with enzymes self-assembled on their surface. The immobilized enzymes on the surface of achromosomal and/or anucleate minicells, has agricultural, industrial, and environmental applications due to their improved stability durability and, reusability. Also, provided are methods for producing and purifying enzyme-immobilized minicells.

Abstract: The present disclosure is generally directed to a composition comprising a minicell and an active agent and to a bacterial minicell comprising an active agent. The compositions are suitable for improving animal health, increasing live stock production, or preventing animal-to-human transmission from both domestic animals and wildlife populations. Disclosed herein are methods of preparing a minicell encapsulating an active agent, delivering an active agent to a subject, and producing an animal feed and/or an animal vaccination for improving animal health and welfare.

Abstract: A system accesses a first digital audio file that includes a plurality of spoken instructions. The system converts the first digital audio file to a first spectrogram image, applies a filter to determine whether an image quality of the first spectrogram image is below a predetermined image quality, and in response, generates a second spectrogram image from the first spectrogram image using a training model. The system converts the second spectrogram image to a second digital audio file and converts the second digital audio file into multiple vectors that each correspond to a particular spoken instruction. The system identifies related vectors and concatenates the related vectors together in order to create a plurality of concatenated vectors. The system generates, using the plurality of concatenated vectors, a third digital audio file that includes concatenated spoken instructions from the first digital audio file.

Abstract: Compositions and methods related to anucelate cells (e.g., bacterial minicells) for pesticide degradation applications including related cells, polypeptides, and vectors.

Abstract: Compositions and methods related to anucelate cells (e.g., bacterial minicells) for pesticide degradation applications including related cells, polypeptides, and vectors.

Abstract: The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.