Patents by Inventor Amy L. Hiddessen
Amy L. Hiddessen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240093280Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.Type: ApplicationFiled: November 27, 2023Publication date: March 21, 2024Inventors: Amy L. HIDDESSEN, Benjamin J. HINDSON
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Patent number: 11866771Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.Type: GrantFiled: June 11, 2021Date of Patent: January 9, 2024Assignee: Bio-Rad Laboratories, Inc.Inventors: Amy L. Hiddessen, Benjamin J. Hindson
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Publication number: 20230383280Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.Type: ApplicationFiled: August 8, 2023Publication date: November 30, 2023Inventors: Lewis A. Marshall, Amy L. Hiddessen, Nathan P. Hoverter, Klint A. Rose, Juan G. Santiago, Matthew S. Munson, Janine Mok, Sean Arin, Yatian Qu, Andrew Lee, Michael Christopher De Renzi
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Publication number: 20230372935Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.Type: ApplicationFiled: July 31, 2023Publication date: November 23, 2023Inventors: Amy L. Hiddessen, Donald A. Masquelier, Kevin D. Ness, Benjamin J. Hindson, Anthony J. Makarewicz, Erin R. Chia, Billy W. Colston, Serge Saxonov, Svilen S. Tzonev, Michael Y. Lucero, Ryan T. Koehler
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Publication number: 20230357748Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.Type: ApplicationFiled: May 1, 2023Publication date: November 9, 2023Inventors: Lewis A. Marshall, Amy L. Hiddessen, Nathan P. Hoverter, Klint A. Rose, Juan G. Santiago
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Patent number: 11674132Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.Type: GrantFiled: August 19, 2020Date of Patent: June 13, 2023Assignee: Purigen Biosystems, Inc.Inventors: Lewis A. Marshall, Amy L. Hiddessen, Nathan P. Hoverter, Klint A. Rose, Juan G. Santiago
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Publication number: 20220008914Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.Type: ApplicationFiled: September 27, 2021Publication date: January 13, 2022Applicant: Bio-Rad Laboratories, Inc.Inventors: Amy L. Hiddessen, Donald A. Masquelier, Kevin D. Ness, Benjamin J. Hindson, Anthony J. Makarewicz, JR., Erin R. Chia, Billy W. Colston, JR., Serge Saxonov, Svilen S. Tzonev, Michael Y. Lucero, Ryan T. Koehler
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Publication number: 20210363574Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.Type: ApplicationFiled: June 11, 2021Publication date: November 25, 2021Inventors: Amy L. HIDDESSEN, Benjamin J. HINDSON
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Publication number: 20210230576Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.Type: ApplicationFiled: April 14, 2021Publication date: July 29, 2021Inventors: Lewis A. MARSHALL, Amy L. HIDDESSEN, Nathan P. HOVERTER, Klint A. ROSE, Juan G. SANTIAGO, Matthew S. MUNSON, Janine MOK, Sean ARIN, Yatian QU, Andrew LEE, Michael Christopher De Renzi
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Patent number: 11060136Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.Type: GrantFiled: June 20, 2019Date of Patent: July 13, 2021Assignee: Bio-Rad Laboratories, Inc.Inventors: Amy L. Hiddessen, Benjamin J. Hindson
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Patent number: 11041150Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.Type: GrantFiled: August 1, 2018Date of Patent: June 22, 2021Assignee: Purigen Biosystems, Inc.Inventors: Lewis A. Marshall, Amy L. Hiddessen, Nathan P. Hoverter, Klint A. Rose, Juan G. Santiago, Matthew S. Munson, Janine Mok, Sean Arin, Yatian Qu, Andrew Lee, Michael Christopher DeRenzi
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Publication number: 20210054361Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.Type: ApplicationFiled: August 19, 2020Publication date: February 25, 2021Inventors: Lewis A. MARSHALL, Amy L. HIDDESSEN, Nathan P. HOVERTER, Klint A. ROSE, Juan G. SANTIAGO
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Patent number: 10822603Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.Type: GrantFiled: July 26, 2018Date of Patent: November 3, 2020Assignee: Purigen Biosystems, Inc.Inventors: Lewis A. Marshall, Amy L. Hiddessen, Nathan P. Hoverter, Klint A. Rose, Juan G. Santiago
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Patent number: 10676778Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.Type: GrantFiled: November 20, 2017Date of Patent: June 9, 2020Assignee: Bio-Rad Laboratories, Inc.Inventors: Adam Lowe, Geoff McDermott, Christopher M. Hindson, Erin R. Chia, Amy L. Hiddessen, Benjamin J. Hindson, Chunxiao Han, Yaqi Wang
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Publication number: 20200086312Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.Type: ApplicationFiled: October 29, 2019Publication date: March 19, 2020Applicant: Bio-Rad Laboratories, Inc.Inventors: Amy L. HIDDESSEN, Donald A. MASQUELIER, Kevin D. NESS, Benjamin J. HINDSON, Anthony J. MAKAREWICZ, JR., Erin R. CHIA, Billy W. COLSTON, JR., Serge SAXONOV, Svilen S. TZONEV, Michael Y. LUCERO, Ryan T. KOEHLER
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Publication number: 20190316191Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.Type: ApplicationFiled: June 20, 2019Publication date: October 17, 2019Inventors: Amy L. HIDDESSEN, Benjamin J. HINDSON
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Patent number: 10415030Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.Type: GrantFiled: October 30, 2018Date of Patent: September 17, 2019Assignee: Purigen Biosystems, Inc.Inventors: Lewis A. Marshall, Amy L. Hiddessen, Nathan P. Hoverter, Klint A. Rose, Juan G. Santiago
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Patent number: 10378048Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.Type: GrantFiled: March 7, 2017Date of Patent: August 13, 2019Assignee: Bio-Rad Laboratories, Inc.Inventors: Amy L. Hiddessen, Benjamin J. Hindson
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Publication number: 20190218593Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.Type: ApplicationFiled: December 10, 2018Publication date: July 18, 2019Inventors: Erin R. CHIA, Amy L. HIDDESSEN, Benjamin J. HINDSON, Adam LOWE, Chunxiao HAN
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Patent number: 10240187Abstract: Method of detecting a signal from droplets for instrument calibration. In the method, a calibration standard for a droplet detection instrument may be received. The calibration standard may have been shipped at least one kilometer when received. The calibration standard may include a mixture of first droplets and second droplets. Each of the first and second droplets may be encapsulated by an immiscible carrier liquid and may have a stabilizing droplet skin that is formed by heating and that includes a skin-forming protein. Each the first droplets may contain a fluorescent dye, and each of the second droplets may contain a fluorescent dye. A fluorescence signal may be detected from a plurality of the first and second droplets with a droplet detection instrument. The fluorescence signal may be stronger for the first droplets than the second droplets.Type: GrantFiled: December 22, 2016Date of Patent: March 26, 2019Assignee: Bio-Rad Laboratories, Inc.Inventors: Amy L. Hiddessen, Erin R. Chia