Abstract: Described herein are methods for the efficient production of a heteromultimeric protein, such as a bispecific antibody. Heteromultimeric proteins may be capable of specifically binding to more than one target molecule or different epitopes on a single target molecule. The methods modulate parameters to improve assembly of the heteromultimeric proteins at higher yield and efficiency than otherwise possible. Also described are compositions comprising a hinge-containing polypeptide, such as a half-antibody.

Abstract: Techniques and system configurations for demand-based product sourcing in an electronic commerce setting is described herein. In one example, information about an item corresponding to a plurality of fulfillment locations is stored in a database. The information includes a plurality of transit times and a plurality of transit costs corresponding to each of the plurality of transit times. A subset of transit times is selected upon receiving a location of a user. A subset of transit costs corresponding with the subset of transit times is selected. An indication of a selection of a transit time is received. The indication includes the item, a quantity of the item, and a destination. A preferred fulfillment location of a subset of fulfillment locations is calculated, the calculating using the transit time, the location, and a selection rule, wherein the selection rule is generated using a plurality of selection attributes.

Abstract: Techniques and system configurations for demand-based product sourcing in an electronic commerce setting is described herein. In one example, information about an item corresponding to a plurality of fulfillment locations is stored in a database. The information includes a plurality of transit times and a plurality of transit costs corresponding to each of the plurality of transit times. A subset of transit times is selected upon receiving a location of a user. A subset of transit costs corresponding with the subset of transit times is selected. An indication of a selection of a transit time is received. The indication includes the item, a quantity of the item, and a destination. A preferred fulfillment location of a subset of fulfillment locations is calculated, the calculating using the transit time, the location, and a selection rule, wherein the selection rule is generated using a plurality of selection attributes.

Abstract: A novel and efficient single pot synthetic schemes are disclosed for preparing modified nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs. The novel method is used to prepare nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs having non-persistent or persistent and non-persistent modifications. Novel biomolecular reactions are also disclosed using the novel modified biomolecules disclosed herein.

Abstract: Modified nucleotides are disclosed for use in single molecule sequencing, methods for making the modified nucleotides and method for using the modified nucleotides. Linkers for making the modified nucleotide are also disclosed.

Abstract: Provided herein are agents that bind to soluble beta-amyloid. Also provided are in vivo and in vitro methods for detecting soluble beta-amyloid in a sample that may include brain tissue.

Abstract: Agents and methods for qualitative and quantitative analysis a protein complex or protein complexes using isotope-labeled symmetrical bifunctional crosslinkers and mass spectrometry are provided. Targeting moieties, cell permeability moieties, or affinity moieties, may be appended to the bifunctional crosslinkers. The isotope-labeled symmetrical bifunctional crosslinkers may be used in a kit or as a library.