Abstract: Process for enriching microalgal biomass, in particular of the genus Nannochloropsis, with different polyunsaturated fatty acids, primarily docosahexaenoic acid (22:6 n-3, DHA), using oils that are rich in these fatty acids. The process comprises the following steps: Preparing a solution or an emulsion of lipids+emulsifying agent (BSA), Preparing the biomass concentrate to obtain a final concentration of mg/ml or greater, Enriching the biomass concentrate by adding the lipids or the emulsion thereof to the microalgal concentrate, Allowing the mixture to rest under constant stirring for at least 24 hours under lighting conditions. The process allows for the simultaneous enrichment of microalgal biomass of the genus Nannochloropsis with eicosapentaenoic acid (20:5 n-3) (EPA) and docosahexaenoic acid (22:6 n-3) (DHA), to obtain a minimum EPA:DHA weight ratio of 10:1, with an EPA content of at least 10% of the total fatty acid content.

Abstract: The invention relates to the cloning, sequencing and characterization of the gene responsible for Quorum Quenching (QQ) activity against Quorum Sensing (QS) signals of the Tenacibaculum sp. strain 20J (CECT7426). Said gene encodes a peptide having at least lactonase activity with a percentage of identity less than 38% with the lactonases described up until now for other species, as well as the sequences of the homologous genes present in other species of the genus Tenacibaculum. Said peptide shows a broad spectrum of activity degrading optionally substituted N-acyl-homoserine lactones (AHLs) of 4-14 carbon atoms in the side chain thereof, is active at pH comprised between 3 and 9, proteinase K- and chymotrypsin-resistant and does not interact with ?-lactam antibiotics.

Abstract: Process for enriching microalgal biomass, in particular of the genus Nannochloropsis, with different polyunsaturated fatty acids, primarily docosahexaenoic acid (22:6 n-3, DHA), using oils that are rich in these fatty acids. The process comprises the following steps: Preparing a solution or an emulsion of lipids+emulsifying agent (BSA), Preparing the biomass concentrate to obtain a final concentration of mg/ml or greater, Enriching the biomass concentrate by adding the lipids or the emulsion thereof to the microalgal concentrate, Allowing the mixture to rest under constant stirring for at least 24 hours under lighting conditions. The process allows for the simultaneous enrichment of microalgal biomass of the genus Nannochloropsis with eicosapentaenoic acid (20:5 n-3) (EPA) and docosahexaenoic acid (22:6 n-3) (DHA), to obtain a minimum EPA:DHA weight ratio of 10:1, with an EPA content of at least 10% of the total fatty acid content.

Abstract: The invention relates to the cloning, sequencing and characterization of the gene responsible for Quorum Quenching (QQ) activity against Quorum Sensing (QS) signals of the Tenacibaculum sp. strain 20J (CECT7426). Said gene encodes a peptide having at least lactonase activity with a percentage of identity less than 38% with the lactonases described up until now for other species, as well as the sequences of the homologous genes present in other species of the genus Tenacibaculum. Said peptide shows a broad spectrum of activity degrading optionally substituted N-acyl-homoserine lactones (AHLs) of 4-14 carbon atoms in the side chain thereof, is active at pH comprised between 3 and 9, proteinase K- and chymotrypsin-resistant and does not interact with ?-lactam antibiotics.

Abstract: Use of bacterial cells of the genus Tenacibaculum, from the crude cellular extract or from its cultivation supernatants, capable of degrading N-acyl homoserine lactones, for quorum quenching, for treatment of infectious bacterial illnesses or to inhibit formation of biofilms.

Abstract: Use of bacterial cells of the genus Tenacibaculum, from the crude cellular extract or from its cultivation supernatants, capable of degrading N-acyl homoserine lactones, for quorum quenching, for treatment of infectious bacterial illnesses or to inhibit formation of biofilms.