Abstract: Luminescence immuno-test kits consist of an antigen or antibody capable of showing luminescence and labelled with phthalic hydrazides, an antibody or antigen preferably bound to a carrier and an oxidizing reagent inducing the luminescence to occur and are characterized in that the oxidizing reagent is a pre-fabricated solution of catalase, optionally stabilized by a bacteriostat, and the initiator is a pre-fabricated peroxide solution which is at least 20 minutes old. These luminescence immuno-tests are particularly stable and may be subjected to a quality control in a simple and inexpensive way.

Abstract: Plastics surfaces are preactivated for immobilizing organo-chemical and biologic materials by coating the surfaces with a polypeptide which consists of a defined composition of hydrophobic amino acids and amino acids having side chains capable of activation and coupling. After activation of the plastics surfaces having been coated in this manner with various coupling reagents, it is possible to firmly fix organo-chemical and biologic materials. The materials having been immobilized in this manner are extremely stable to various reaction conditions.

Abstract: Luminescence immunoassays for haptens can be improved and made more sensitive by using a luminescence labelled hapten conjugate which contains as the linkage group a chain-like polymer having repeating functional groups having bound thereto per mole both a plurality of moles of groups capable of luminescence and a plurality of moles of hapten. The antibody used is preferably one which is prepared by the use of another chain-like polymer having bound thereto the hapten by a different chemical reaction.

Abstract: 1. Immunological method for the determination of free substances having hapten properties.2.1. Immunological methods for the determination of free substances having hapten properties, especially of free thyroid hormones, are based on various determination principles, all of which either have disadvantages from the practical viewpoint or, by reason of the basic principle of the method, provide limited information in marginal cases. The new method is intended to simplify and improve the routine clinical determination of free substances, because of the avoidance of sources of error associated with known methods, while having a procedure which is straightforward for practical use.2.2.