Abstract: Herein are provided a method for producing a multispecific antibody comprising the steps of providing a mammalian cell expressing the antibody, transfecting said mammalian cell with an expression vector comprising an expression cassette encoding a polypeptide of the antibody that has a domain crossover, cultivating the transfected cell and recovering the antibody from the cell or the cultivation medium and thereby producing the multispecific antibody.

Abstract: Herein are provided a method for producing a multispecific antibody comprising the steps of providing a mammalian cell expressing the antibody, transfecting said mammalian cell with an expression vector comprising an expression cassette encoding a polypeptide of the antibody that has a domain crossover, cultivating the transfected cell and recovering the antibody from the cell or the cultivation medium and thereby producing the multispecific antibody.

Abstract: Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.

Abstract: Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.

Abstract: Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.

Abstract: It is reported herein a method for the determination of the amount of immunoglobulin-encoding mRNA comprising: a) providing a sample, b) performing a polymerase chain reaction for amplifying the light chain with the primers of SEQ ID NO: and 24 and the probe of SEQ ID NO: 33, and/or c) performing a polymerase chain reaction for amplifying the heavy chain with the primers of SEQ ID NO: 19 and 21 and the probe of SEQ ID NO: 40, and d) quantitating with an efficiency of 2.0. The primers with SEQ ID NOs 23 and 24 bind at positions CL 247-266 and CL166-185, respectively, and the probe with SEQ ID NO: 33 binds at 189-212 in human IgG koppa chain. The primer with SEQ ID NO: 19 binds at CH region 2 position 220-237 and the primer with SEQ ID NO: 21 binds at CH region 3 position 114-133. Finally the probe with SEQ ID NO: 40 binds from position 315 in CH2 to position 7 in CH3.