Abstract: The present disclosure relates generally to bacterial NADH oxidases and, more particularly, to novel NADH oxidases obtained from Lactobacillus plantarum, and derivatives thereof that demonstrate enzymatic activity for NADH, NADPH, or both NADH and NADPH. The compositions comprising an NADH oxidase obtained from L. plantarum or derivatives thereof include: isolated enzymes; recombinantly produced enzymes and derivatives thereof, as well as catalytically active portions thereof; nucleic acids encoding an NADH oxidase obtained from L. plantarum, derivatives thereof, and portions thereof. The methods of the present invention include isolation of NADH oxidases obtained from L. plantarum, derivatives thereof, and portions thereof, and methods for enzymatic reactions comprising NADH oxidase obtained from L. plantarum, including the production of enantiomer-enriched organic compounds.

Abstract: The present disclosure relates generally to bacterial NADH oxidases and, more particularly, to novel NADH oxidases obtained from Lactobacillus plantarum, and derivatives thereof that demonstrate enzymatic activity for NADH, NADPH, or both NADH and NADPH. The compositions comprising an NADH oxidase obtained from L. plantarum or derivatives thereof include: isolated enzymes; recombinantly produced enzymes and derivatives thereof, as well as catalytically active portions thereof; nucleic acids encoding an NADH oxidase obtained from L. plantarum, derivatives thereof, and portions thereof. The methods of the present invention include isolation of NADH oxidases obtained from L. plantarum, derivatives thereof, and portions thereof, and methods for enzymatic reactions comprising NADH oxidase obtained from L. plantarum, including the production of enantiomer-enriched organic compounds.

Abstract: A whole cell catalyst is described comprising a hydantoinase, a racemase and a carbamoylase. Thus this catalyst is able to degrade hydantoins directly into the amino acids. Additionally, a process for the production of this catalysts and for the production of amino acids is claimed.

Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.

Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.

Abstract: The present invention is directed to compositions and methods comprising NAD(P)H oxidases, particularly bacterial oxidases, nucleic acids, recombinant plasmid vectors and recombinant proteins therein encoded, and host cells comprising the oxidases and nucleic acids. The present invention also comprises an isolated bacterial oxidase that oxidizes both NADH and NADPH. Methods for producing the enzymes and enzymatic reactions comprising use of NAD(P)H oxidases and products of such reactions are also disclosed.

Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.

Abstract: Compositions and methods of using a carbonyl reductase from Lactococcus lactis (LLCR or LL-CR) are provided. One aspect provides an isolated polypeptide from Lactococcus lactis, wherein the polypeptide is an (R)-specific carbonyl reductase or a fragment thereof and reduces acetophenone or alph,beta-unsaturated carbonyl compound to form a reduced R enantiomeric product. In certain aspects, LLCR is stable in from about 1.0% to about 60% organic solvent or more than 10%, 20%, 30%, 40% or 50% organic solvent.

Abstract: The present invention relates to an amidase enzyme, nucleic acids encoding the amidase, as well as methods of employing the nucleic acids and/or amidase to produce, for example, enantiomericallyenriched compounds such as D-amino acids.

Abstract: The instant invention is directed to a rec-hydantoin-racemase from Arthrobacter aurescens DSM 3747. Furthermore, the gene encoding for the racemase and plasmids, vectors and microorganisms comprising this gene are to be protected. Use in a process for the production of amino carboxylic acids or derivatives thereof.

Abstract: The present invention is directed to compositions and methods comprising NAD(P)H oxidases, particularly bacterial oxidases, nucleic acids, recombinant plasmid vectors and recombinant proteins therein encoded, and host cells comprising the oxidases and nucleic acids. The present invention also comprises an isolated bacterial oxidase that oxidizes both NADH and NADPH. Methods for producing the enzymes and enzymatic reactions comprising use of NAD(P)H oxidases and products of such reactions are also disclosed.

Abstract: A molecular weight-enlarged, homogeneously soluble ligand, useful in catalysts, particularly for the synthesis of enantiomerically enriched organic compounds, includes: a weight average molecular weight of at least 1000 g/mol; a molecular weight-enlarging polymer; optionally, a polymer linker; and at least one homochiral active center; wherein the active center is bound to the molecular weight-enlarging polymer through the polymer linker or is bound directly to the molecular weight-enlarging polymer.

Abstract: The present invention relates to a new D-carbamoylase and the gene sequences which code for this from the organism Arthrobacter crystallopoietes DSM 20117. Plasmids, vectors, microorganisms, particular primers and specific possible uses of the enzymes according to the invention are also mentioned. The invention moreover describes a new process for the discovery of enzymes which can be employed in a process for the preparation of D-amino acids starting from 5′-substituted hydantoins.

Abstract: The invention relates to the use of the N-acetyl amino acid racemase from Amycolatopsis orientalis subspecies lurida for the racemiszation of N-carbamoyl amino acids. This use permits the 100% preparation of optically pure amino acids starting from racemic hydantoins in an enzymatic overall process.

Abstract: The first embodiment of the present invention provides a process, which includes: in a continuous process in a membrane reactor, asymmetrically hydrogenating at least one C═C, C═N or C═O double bond with a catalyst. Another embodiment of the present invention provides a ligand, which includes at least one di-1,3-aminophosphine homochiral active center; optionally, a linker; and a molecular weight-enlarging polymer; wherein the active center is bound to the molecular weight-enlarging polymer through the linker or is bound directly to the molecular weight-enlarging polymer; and wherein the linker is defined in the claims. Another embodiment of the present invention provides a process for preparing the above-noted ligand, and a catalyst that includes the above-noted ligand.

Abstract: A microorganism is described which is transformed with DNAs which encode a hydantoinnase, a racemase, and a carbamoylase. As a result, the microorganism is able to degrade hydantoins directly to amino acids. A process for the production of the microorganism and a process for producing amino acids with the microorganism is also described.

Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.

Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.

Abstract: The present invention relates to an amidase enzyme, nucleic acids encoding the amidase, as well as methods of employing the nucleic acids and/or amidase to produce, for example, enantiomericallyenriched compounds such as D-amino acids.

Abstract: Molecular weight-enlarged, homogeneously soluble ligands are provided that are especially useful for hydrogenation catalysts, wherein the ligands contain homochiral active centers of bis(3,4-diarylphosphinyl)pyrrolidines, and their use in producing cataylsts for enantioselective reactions, as well as the catalysts thus produced.