Patents by Inventor Andrei Miasnikov
Andrei Miasnikov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20160177319Abstract: Described are methods for increasing the expression of a transgene in eukaryotic cells by reducing RNA interference (RNAi), and variant cells produce by the method. The methods and variant cells are useful, for example, for the efficient production of therapeutic and industrial polypeptides in eukaryotic cells.Type: ApplicationFiled: July 31, 2014Publication date: June 23, 2016Applicant: DANISCO US INC.Inventor: Andrei MIASNIKOV
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Patent number: 9296850Abstract: It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has ?13C value of greater than ?22%.Type: GrantFiled: January 26, 2015Date of Patent: March 29, 2016Assignees: The Goodyear Tire & Rubber Company, Danisco US, Inc.Inventors: Frank J. Feher, Gregory M. Whited, Gopal K. Chotani, Fernando Valle, Carol Fioresi, Karl J. Sanford, Joseph McAuliffe, Marguerite Cervin, Aaron S. Puhala, Andrei Miasnikov, Ilana S. Aldor
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Patent number: 9273295Abstract: The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.Type: GrantFiled: September 14, 2011Date of Patent: March 1, 2016Assignee: DUPONT NUTRITION BIOSCIENCES APSInventors: Andrei Miasnikov, Vijay Kumar, Oliver Kensch, Klaus Pellangahr, Birgitta Leuthner, Ulrich Kettling, Andre Koltermann
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Patent number: 9260727Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.Type: GrantFiled: August 30, 2012Date of Patent: February 16, 2016Assignees: DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANYInventors: Marguerite A. Cervin, Gopal K. Chotani, Frank J. Feher, Richard La Duca, Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Aaron S. Puhala, Karl J. Sanford, Fernando Valle, Gregory M. Whited
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Publication number: 20160040144Abstract: Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and Trichoderma host cells having a modification comprising or consisting essentially of disruption or deletion of nucleotide(s) for expression of this cellulose, whereby EGV expression is prevented.Type: ApplicationFiled: August 20, 2015Publication date: February 11, 2016Applicant: DANISCO US INC.Inventors: Andrei Miasnikov, Michael Schelle, Michael Ward
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Patent number: 9187738Abstract: The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position and at a location in an external loop distal to the active site of the enzyme which is more hydrophilic; or c) a substitution or insertion at one or more of positions disclosed herein or a deletion at one or more positions disclosed herein. The invention also relates to polypeptide produced by the method and to novel nucleic acids.Type: GrantFiled: December 19, 2011Date of Patent: November 17, 2015Assignee: DUPONT NUTRITION BIOSCIENCES APSInventors: Andrei Miasnikov, Richard R. Bott, Jens Frisbæk Sørensen
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Publication number: 20150203620Abstract: It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has ?13C value of greater than ?22%.Type: ApplicationFiled: January 26, 2015Publication date: July 23, 2015Applicants: DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANYInventors: Frank J. Feher, Gregory M. Whited, Gopal K. Chotani, Fernando Valle, Carol Fioresi, Karl J. Sanford, Joseph McAuliffe, Marguerite Cervin, Aaron S. Puhala, Andrei Miasnikov, Ilana S. Aldor
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Publication number: 20150191712Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.Type: ApplicationFiled: November 17, 2014Publication date: July 9, 2015Inventors: Richard R. BOTT, Marguerite A. Cervin, James T. Kellis, JR., Joseph C. Mcauliffe, Andrei Miasnikov, Caroline M. Peres, Christopher L. Rife, Derek H. Wells, Walter Weyler, Gregory M. Whited
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Publication number: 20150044736Abstract: Described are compositions and methods relating to a fungal cutinase cloned from Magnaporthe grisea, polynucleotides encoding the cutinase, and methods of use thereof. The compositions and methods have particular application in detergent cleaning compositions and synthesis reactions.Type: ApplicationFiled: July 23, 2014Publication date: February 12, 2015Applicant: DANISCO US INC.Inventors: Christian D. Adams, Andrei Miasnikov
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Patent number: 8940849Abstract: It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has ?13C value of greater than ?22‰.Type: GrantFiled: March 11, 2013Date of Patent: January 27, 2015Assignees: The Goodyear Tire & Rubber Company, Danisco US Inc.Inventors: Frank J. Feher, Gregory M. Whited, Gopal K. Chotani, Fernando Valle, Carol Fioresi, Karl J. Sanford, Joseph McAuliffe, Marguerite Cervin, Aaron S. Puhala, Andrei Miasnikov, Ilana S. Aldor
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Patent number: 8916370Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.Type: GrantFiled: March 30, 2012Date of Patent: December 23, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Richard R. Bott, Marguerite A. Cervin, James T. Kellis, Jr., Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Christopher Lee Rife, Derek H. Wells, Walter Weyler, Gregory M. Whited
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Patent number: 8889371Abstract: The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Corynebacterium species.Type: GrantFiled: February 1, 2010Date of Patent: November 18, 2014Assignee: Dupont Nutrition Biosciences APSInventors: Andrei Miasnikov, Jørn Borch Søe, Jørn Dalgaard Mikkelsen, Mira Povelainen, Virve Pitkanen
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Patent number: 8709785Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.Type: GrantFiled: August 30, 2012Date of Patent: April 29, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Marguerite A. Cervin, Gopal K. Chotani, Frank J. Feher, Richard La Duca, Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Aaron S. Puhala, Karl J. Sanford, Fernando Valle, Gregory M. Whited
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Publication number: 20140024098Abstract: The invention provides methods for expression of a catalase enzyme in a Trichoderma host cell. In one embodiment, the catR gene from Aspergillus niger is expressed in Trichoderma reesei, resulting in improved yields of catalase enzyme in comparison with expression of catR in A. niger.Type: ApplicationFiled: July 19, 2013Publication date: January 23, 2014Applicant: Danisco US Inc.Inventors: Timothy C. Dodge, Katherine Marie Hoffmann, Andrei Miasnikov, Michael Ward
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Publication number: 20130260432Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.Type: ApplicationFiled: March 30, 2012Publication date: October 3, 2013Applicant: Danisco US Inc.Inventors: Richard R. BOTT, Marguerite A. Cervin, James T. Kellis, JR., Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Christopher Lee Rife, Derek H. Wells, Walter Weyler, Gregory M. Whited
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Publication number: 20130253141Abstract: It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has ?13C value of greater than ?22‰.Type: ApplicationFiled: March 11, 2013Publication date: September 26, 2013Applicants: DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANYInventors: Frank J. Feher, Gregory M. Whited, Gopal K. Chotani, Fernando Valle, Carol Fioresi, Karl J. Sanford, Joseph McAuliffe, Marguerite Cervin, Aaron S. Puhala, Andrei Miasnikov, Ilana S. Aldor
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Patent number: 8535900Abstract: The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Corynebacterium species.Type: GrantFiled: February 1, 2010Date of Patent: September 17, 2013Assignee: Dupont Nutrition Biosciences APSInventors: Andrei Miasnikov, Jørn Borch Søe, Jørn Dalgaard Mikkelsen, Mira Povelainen, Virve Pitkanen
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Publication number: 20130136825Abstract: The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.Type: ApplicationFiled: September 14, 2011Publication date: May 30, 2013Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Andrei Miasnikov, Vijay Kumar, Oliver Kensch, Klaus Pellangahr, Birgitta Leuthner, Ulrich Kettling, Andre Koltermann
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Patent number: 8450098Abstract: The method disclosed herein, relates generally to introducing molecules such as biomolecules (e.g., nucleic acids) into a filamentous fungus. More specifically, the methods disclosed herein relate to introducing one or more nucleic acids into a filamentous fungus.Type: GrantFiled: May 21, 2008Date of Patent: May 28, 2013Assignee: Danisco US Inc.Inventors: Steven Kim, Andrei Miasnikov
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Patent number: 8420759Abstract: It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has ?13C value of greater than ?22‰.Type: GrantFiled: June 30, 2009Date of Patent: April 16, 2013Assignees: The Goodyear Tire & Rubber Company, Danisco US, Inc.Inventors: Frank J. Feher, Gregory M. Whited, Gopal K. Chotani, Fernando Valle, Carol Fioresi, Karl J. Sanford, Joseph C. McAuliffe, Marguerite Cervin, Aaron S. Puhala, Andrei Miasnikov, Ilana S. Aldor