Abstract: Systems and methods where a lighting configuration of a vision system is determined using a controllable lighting system and at least one image evaluation tool. The lighting configuration is usable to obtain a desired inspection image of at least one feature of a workpiece Base images are obtained using actual illumination settings of the controllable lighting system. Simulated or synthetic sets image results are generated, based on base images and synthetic lighting configurations. The synthetic lighting configurations include at least one illumination setting which is different from the actual illumination settings used to obtain one or more component base images. A best actual or synthetic lighting configuration is chosen based on the best corresponding set of image results.

Abstract: A system for collecting images at multiple wavelengths for image analysis of biological specimens. The process separates contrasts for images of a biological specimen prepared with a chromogen. Light wavelengths matched with each chromogen optimize separation for each image. Computing attenuation coefficients and extinction coefficients for each chromogen provides for determination of the concentration of each chromogen. A chromogen separator allows for subtracting predetermined chromogens to generate new images and aid in identification of cell morphology. The system can perform the image analysis of the new images using image processing techniques such as segmentation, feature calculation and object classification. At each stage of image processing, data from each of the new images may influence the processing of the other images.

Abstract: Systems and methods where a lighting configuration of a vision system is determined using a controllable lighting system and at least one image evaluation tool. The lighting configuration is usable to obtain a desired inspection image of at least one feature of a workpiece Base images are obtained using actual illumination settings of the controllable lighting system. Simulated or synthetic sets image results are generated, based on base images and synthetic lighting configurations. The synthetic lighting configurations include at least one illumination setting which is different from the actual illumination settings used to obtain one or more component base images. A best actual or synthetic lighting configuration is chosen based on the best corresponding set of image results.