Abstract: A method for attaching a prepared sample to a carrier in a focused ion beam chamber. The method includes reducing a temperature within the chamber to substantially below room temperature followed by moving the prepared sample adjacent to a substrate carrier surface. The temperature can be lowered sufficiently to establish a cryogenic condition in the chamber. Attachment of the prepared sample to the substrate carrier is done by controlling the focused ion beam to raster a target area of the surface in the absence of a gas deposition precursor, to sputter material onto the base of the sample and the substrate carrier surface, thereby binding the prepared sample to the substrate carrier.

Abstract: The invention provides methods of screening for capsid-encoding nucleotide sequences of adeno-associated virus (“AAV”) particles capable of infecting a subject's neurons, or sub-compartments thereof, involving neurons derived from induced pluripotent stem cells or embryonic stem cells. The invention also provides AAV capsids, capsid-encoding nucleotide sequences, expression vectors, viral particles, cells and kits for use in methods of treating neuromuscular or neuromotor disorders, such as spasticity. Sequences generated from the methods may lead to new gene therapies targeted to select populations of neurons, such as motor neurons innervating muscle cells, thus providing high specificity that may be personalised to the subject.

Abstract: A carrier grid with integrated gas delivery system for use in a charged particle beam system (CPB). The carrier grid has a body with an internal reservoir for storing a gas. A post extends from the body with an end for supporting a sample to be operated upon, and an outlet tip extends from the end of the post. A channel extends from the reservoir, through the post and ends in the outlet tip, where the outlet tip seals the stored gas in the body. Cutting the outlet tip near its base, with a focused ion beam (FIB) by example, will open the channel to the CPB chamber, allowing the prestored gas within the reservoir to escape. A FIB or electron beam directed at the junction of the sample positioned near the post will cause deposition and subsequent attachment of the sample to the post in presence of the gas.

Abstract: A vehicle control system for a vehicle, the vehicle control system comprising a controller, wherein the controller has an activatable secondary parked mode in which the controller automatically controls a plurality of components of the vehicle, each component also being controllable by a corresponding user control, and activation of the secondary parked mode changes the state of each component from an in-use state to a parked state when the vehicle attends a delivery or collection.

Abstract: A notification can be generated to notify a user of a value of a first parameter of a vehicle. Generating the notification includes identifying a second parameter based on an association of the second parameter with the first parameter; comparing the value of the first parameter to a first threshold value and a second threshold value; and generating the notification notifying the user of the value of the first parameter based on a value of the second parameter if the value of the first parameter is between the first and second thresholds.

Abstract: A carrier grid with integrated gas delivery system for use in a charged particle beam system (CPB). The carrier grid has a body with an internal reservoir for storing a gas. A post extends from the body with an end for supporting a sample to be operated upon, and an outlet tip extends from the end of the post. A channel extends from the reservoir, through the post and ends in the outlet tip, where the outlet tip seals the stored gas in the body. Cutting the outlet tip near its base, with a focused ion beam (FIB) by example, will open the channel to the CPB chamber, allowing the prestored gas within the reservoir to escape. A FIB or electron beam directed at the junction of the sample positioned near the post will cause deposition and subsequent attachment of the sample to the post in presence of the gas.

Abstract: A novel method for cross-section sample preparation has a sample oriented normal to an SEM/GFIS or other imaging column via a stage, and is operated upon by an FIB to form the cross-section pre-lamella within the sample, followed by an approximate 90° rotation with no tilt of the stage for cut out by the FIB. Asymmetric trenches are milled to have a three-dimensional depth profile to ensure that the FIB has clear line of sight to a face of the resulting pre-lamella when the sample has been rotated. The three-dimensional depth profile further minimizes overall milling time required for the preparation of the pre-lamella.

Abstract: An assembly for a motor vehicle is provided. The assembly comprises a steering wheel assembly comprising a steering wheel hub for coupling to a steering column of the motor vehicle, such that rotation of the hub about a steering axis rotates the steering column, and a steering wheel rim assembly comprising a steering wheel rim extending around a first axis of the steering wheel assembly, wherein the steering wheel rim is movably coupled to the steering wheel hub, such that the steering wheel rim is selectively pivotable relative to the steering wheel hub about a second axis, the second axis having a component in a direction perpendicular to the first axis, and a controller configured to determine a pivot angle of the steering wheel rim and activate or deactivate an ignition system and/or a driver restraint system of the vehicle based on the pivot angle.

Abstract: A notification can be generated to notify a user of a value of a first parameter of a vehicle. Generating the notification includes identifying a second parameter based on an association of the second parameter with the first parameter; comparing the value of the first parameter to a first threshold value and a second threshold value; and generating the notification notifying the user of the value of the first parameter based on a value of the second parameter if the value of the first parameter is between the first and second thresholds.

Abstract: A method of tilting a vehicle is provided, and a vehicle is provided. The vehicle has a tilt control system to control the tilt of the vehicle. The method includes tilting the tilting vehicle and/or another tilting vehicle in response to at least one external trigger.

Abstract: A novel method for cross-section sample preparation has a sample oriented normal to an SEM/GFIS or other imaging column via a stage, and is operated upon by an FIB to form the cross-section pre-lamella within the sample, followed by an approximate 90° rotation with no tilt of the stage for cut out by the FIB. Asymmetric trenches are milled to have a three-dimensional depth profile to ensure that the FIB has clear line of sight to a face of the resulting pre-lamella when the sample has been rotated. The three-dimensional depth profile further minimizes overall milling time required for the preparation of the pre-lamella.

Abstract: An assembly for a motor vehicle is provided. The assembly comprises a steering wheel assembly comprising a steering wheel hub for coupling to a steering column of the motor vehicle, such that rotation of the hub about a steering axis rotates the steering column, and a steering wheel rim assembly comprising a steering wheel rim extending around a first axis of the steering wheel assembly, wherein the steering wheel rim is movably coupled to the steering wheel hub, such that the steering wheel rim is selectively pivotable relative to the steering wheel hub about a second axis, the second axis having a component in a direction perpendicular to the first axis, and a controller configured to determine a pivot angle of the steering wheel rim and activate or deactivate an ignition system and/or a driver restraint system of the vehicle based on the pivot angle.

Abstract: A vehicle control system for a vehicle, the vehicle control system comprising a controller, wherein the controller has an activatable secondary parked mode in which the controller automatically controls a plurality of components of the vehicle, each component also being controllable by a corresponding user control, and activation of the secondary parked mode changes the state of each component from an in-use state to a parked state when the vehicle attends a delivery or collection.

Abstract: A roll cover assembly for covering a cargo space of a vehicle includes a cassette housing an extendable/retractable roll cover, a first receptacle adjacent a first side wall of the cargo space and having a channel configured to slidably guide a first end of the cassette during insertion or removal, a second receptacle adjacent a second side wall of the cargo space and having an opening configured to receive a second end of the cassette and permit pivoting of the cassette about the second end during removal of the first end from the first receptacle, a locking mechanism releasably locking the first end of the cassette into the first receptacle, and a release device actuatable to release the locking mechanism by pulling along a line-of-action parallel with a direction in which the first end of the cassette moves during removal from the first receptacle.

Abstract: A vehicle loadspace cover is provided that is securable to a portion of a vehicle. The loadspace cover comprises a cargo fixing point configured to secure an item of cargo to the loadspace cover.

Abstract: A method of tilting a vehicle is provided, and a vehicle is provided. The vehicle has a tilt control system to control the tilt of the vehicle. The method includes tilting the tilting vehicle and/or another tilting vehicle in response to at least one external trigger.

Abstract: A handle assembly for opening and closing a vehicle door wherein the handle assembly includes: a recess in an exterior surface of the vehicle door; a cover over the recess wherein the cover is coupled to the vehicle door and is moveable between an open position and a closed position; and a handle member coupled to the cover and retained in the recess when the cover is in the closed position and released from the recess when the cover is in the open position.

Abstract: A lift assembly for a vehicle includes a bracket configured to be fixed to the vehicle. A lift arm is pivotally coupled to the bracket. A mating structure is supported by the lift arm. The mating structure is configured to lift a personal mobility device and includes an electrical connector for electrically mating to an electrical connector of the personal mobility device.

Abstract: A head restraint includes a central part, a fastener for fastening the central part to a backrest of a vehicle seat, and at least one side wing. The side wing is mounted pivotably in relation to the central part between the neutral position and at least one comfort position, and is fixable at least in the comfort position. The fastener is designed to mount the central part pivotably relative to the backrest about a horizontal pivot axis. The central part is pivotable between a parking position and at least one supporting position and is fixable at least in the supporting position.

Abstract: A two part closure for a combo chargeport for use on an electric vehicle is provided. The closure comprises: an inner part including a dust cover for a DC chargeport; and an outer part configured to cover the AC chargeport. The inner and outer parts are hingeably attached to the vehicle such that the closure can be closed with a single action.