Abstract: Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

Abstract: A portable temperature-controlled container for receiving and housing one or more handheld carriers, each handheld carrier configured to transfer samples to and from a temperature-controlled storage environment, the handheld carrier including a handle and a tray portion, the tray portion configured to be slid into a port of a rack or tower provided in the temperature-controlled storage environment in order to withdraw a sample located in the port, the portable temperature-controlled container including a housing having an opening forming an internal cavity configured to receive one or more handheld carriers, and a lid configured to substantially close the opening, where the housing includes a recess configured to receive the handle of the handheld carrier such that closing of the lid substantially seals the internal cavity when the one or more handheld carriers are placed in the housing.

Abstract: The present disclosure involves systems, software, and computer implemented methods for automatically modifying values of content elements in frames of a video. One example method includes obtaining an input video. A set of elements included in one or more frames of the input video is identified. Metadata is generated for each element in the set of elements. A determination is made that a value for a particular element in the set of elements is targeted for replacement. A replacement value is determined for the particular element and updated metadata is generated. A metadata entry for the particular element is updated to include the determined replacement value. Output content is generated based on the updated metadata the output content is provided for display.

Abstract: A portable temperature-controlled container for receiving and housing one or more handheld carriers, each handheld carrier configured to transfer samples to and from a temperature-controlled storage environment, the handheld carrier including a handle and a tray portion, the tray portion configured to be slid into a port of a rack or tower provided in the temperature-controlled storage environment in order to withdraw a sample located in the port, the portable temperature-controlled container including a housing having an opening forming an internal cavity configured to receive one or more handheld carriers, and a lid configured to substantially close the opening, where the housing includes a recess configured to receive the handle of the handheld carrier such that closing of the lid substantially seals the internal cavity when the one or more handheld carriers are placed in the housing.

Abstract: A system is provided for transporting, handling and monitoring samples in a temperature-controlled storage environment. The system includes a handheld carrier configured to transfer samples to and from a temperature-controlled storage station and a temperature-controlled container for receiving and housing one or more carriers. The carrier includes an integrated sample identification and temperature sensing capability configured to monitor a thermal history of one or more samples during transport, handling and storage including as the samples are conveyed between the temperature-controlled storage environment and the temperature-controlled container. That is, the carrier is adapted to be held in the hand during use. A carrier for conveying and monitoring samples during transport, handling and storage is also provided.

Abstract: A device for indicating violation of a temperature limit of an item is provided. The device is positionable near the item and includes a sensor configured to change its state permanently when the temperature limit is violated. The change in sensor state is machine-readable to provide a permanent indication of the temperature limit violation. The sensor includes a reservoir for storing a fluid that changes phase from a solid to a liquid when the temperature limit is violated and a channel in fluid communication with the reservoir for receiving flow of the fluid in liquid phase from the reservoir. The sensor is arranged to allow flow of the fluid in the liquid phase such that the state of the sensor is permanently changed. The sensor is further configured to prevent the fluid flow from permanently changing the sensor state until activation of the sensor from a resting state. A system and method for detecting violation of a temperature limit of an item using the device is also provided.

Abstract: A biological reaction apparatus for receiving at least one substrate having a sample located in a sample region, and a separate cover, such that a reaction chamber is formed between the cover and substrate over the sample region, wherein the apparatus includes a locating means to locate the substrate; a cover locating means for locating and moving the cover with respect to the substrate; a fluid dispensing means for dispensing fluid into the reaction chamber; and a draining mechanism; wherein the draining mechanism includes wicking means.

Abstract: A biological reaction apparatus for receiving at least one substrate having a sample located in a sample region, and a separate cover, such that a reaction chamber is formed between the cover and substrate over the sample region, wherein the apparatus includes a locating means to locate the substrate; a cover locating means for locating and moving the cover with respect to the substrate; a fluid dispensing means for dispensing fluid into the reaction chamber; and a draining mechanism; wherein the draining mechanism includes wicking means.

Abstract: The present disclosure involves systems, software, and computer implemented methods for automatically modifying values of content elements in frames of a video. One example method includes obtaining an input video. A set of elements included in one or more frames of the input video is identified. Metadata is generated for each element in the set of elements. A determination is made that a value for a particular element in the set of elements is targeted for replacement. A replacement value is determined for the particular element and updated metadata is generated. A metadata entry for the particular element is updated to include the determined replacement value. Output content is generated based on the updated metadata the output content is provided for display.

Abstract: A wearable device is provided for wirelessly reading data in a temperature-controlled environment, the wearable device being adapted to be worn on an upper limb of a user, wherein the wearable device includes an antenna and circuitry adapted to activate the antenna and wirelessly read data from a machine-readable tag in the temperature-controlled environment.

Abstract: A system is provided for transporting, handling and monitoring samples in a temperature-controlled storage environment. The system includes a handheld carrier configured to transfer samples to and from a temperature-controlled storage station and a temperature-controlled container for receiving and housing one or more carriers. The carrier includes an integrated sample identification and temperature sensing capability configured to monitor a thermal history of one or more samples during transport, handling and storage including as the samples are conveyed between the temperature-controlled storage environment and the temperature-controlled container. That is, the carrier is adapted to be held in the hand during use. A carrier for conveying and monitoring samples during transport, handling and storage is also provided.

Abstract: A device for indicating violation of a temperature limit of an item is provided. The device is positionable near the item and includes a sensor configured to change its state permanently when the temperature limit is violated. The change in sensor state is machine-readable to provide a permanent indication of the temperature limit violation. The sensor includes a reservoir for storing a fluid that changes phase from a solid to a liquid when the temperature limit is violated and a channel in fluid communication with the reservoir for receiving flow of the fluid in liquid phase from the reservoir. The sensor is arranged to allow flow of the fluid in the liquid phase such that the state of the sensor is permanently changed. The sensor is further configured to prevent the fluid flow from permanently changing the sensor state until activation of the sensor from a resting state. A system and method for detecting violation of a temperature limit of an item using the device is also provided.

Abstract: A biological reaction apparatus for receiving at least one substrate having a sample located in a sample region, and a separate cover, such that a reaction chamber is formed between the cover and substrate over the sample region, wherein the apparatus includes a locating means to locate the substrate; a cover locating means for locating and moving the cover with respect to the substrate; a fluid dispensing means for dispensing fluid into the reaction chamber; and a draining mechanism; wherein the draining mechanism includes wicking means.

Abstract: A biological reaction apparatus for receiving at least one substrate having a sample located in a sample region, and a separate cover, such that a reaction chamber is formed between the cover and substrate over the sample region, wherein the apparatus includes a locating means to locate the substrate; a cover locating means for locating and moving the cover with respect to the substrate; a fluid dispensing means for dispensing fluid into the reaction chamber; and a draining mechanism; wherein the draining mechanism includes wicking means.

Abstract: An apparatus for imparting an organoleptic quality to a recipient product using a sensate substance obtained from a donor product, the apparatus comprising a donor product storage chamber; and a recipient product storage chamber, the apparatus being arranged to allow a fluid to circulate repeatedly between the donor product storage chamber and the recipient product storage chamber so that at least one sensate substance obtained from the donor product is conveyed from the donor product storage chamber into the recipient product storage chamber and into contact with the recipient product.

Abstract: An method for imparting an organoleptic quality to a tobacco industry product using a sensate substance obtained from a donor product, the method comprising repeatedly circulating a fluid in a closed loop through a donor product storage chamber containing a donor product and a recipient product storage chamber containing a batch of tobacco industry product so that at least one sensate substance obtained from the donor product is conveyed from the donor product storage chamber into the recipient product storage chamber and into contact with the tobacco industry product to impart an organoleptic quality thereto.

Abstract: An method for imparting an organoleptic quality to a tobacco industry product using a sensate substance obtained from a donor product, the method comprising repeatedly circulating a fluid in a closed loop through a donor product storage chamber containing a donor product and a recipient product storage chamber containing a batch of tobacco industry product so that at least one sensate substance obtained from the donor product is conveyed from the donor product storage chamber into the recipient product storage chamber and into contact with the tobacco industry product to impart an organoleptic quality thereto.

Abstract: A cover for a substrate including: a body defining a cavity, for positioning over the substrate to form a reaction chamber; and a projection extending from the body to define a fluid reservoir, when the cover is fitted to the substrate, the fluid reservoir being in fluid communication with the cavity.

Abstract: A biological reaction apparatus for receiving at least one substrate having a sample located in a sample region, and a separate cover, such that a reaction chamber is formed between the cover and substrate over the sample region. The apparatus includes a substrate locator for locating the substrate; a cover locator for locating and moving the cover with respect to the substrate; a fluid dispenser for dispensing fluid into the reaction chamber; and a draining mechanism which includes a wick.

Abstract: A method and apparatus for impregnating tobacco industry products with sensate constituents of botanicals by storing the tobacco industry products and the botanicals separately and applying heat and/or pressure to the apparatus to obtain a modified taste and aroma profile, are disclosed.