Patents by Inventor Angela DeLucia
Angela DeLucia has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240093164Abstract: The present invention relates to a polymerase enzyme from 9° N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.Type: ApplicationFiled: November 27, 2023Publication date: March 21, 2024Inventors: Jerzy OLEJNIK, Angela DELUCIA
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Patent number: 11866741Abstract: The present invention relates to a polymerase enzyme from 9° N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.Type: GrantFiled: June 17, 2021Date of Patent: January 9, 2024Assignee: IsoPlexis CorporationInventors: Jerzy Olejnik, Angela Delucia
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Patent number: 11821032Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: August 21, 2020Date of Patent: November 21, 2023Assignee: IsoPlexis CorporationInventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Publication number: 20230340583Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: April 6, 2023Publication date: October 26, 2023Inventors: Luisa ANDRUZZI, Jimmy ADEDIRAN, Timothy PELLETIER, Austin RICKER, Angela DELUCIA, Ben MCNALLY, Dona HEVRONI, Mong Sano MARMA, John Andrew SHERIDAN
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Publication number: 20220315995Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: February 3, 2022Publication date: October 6, 2022Inventors: Mong Sano MARMA, Luisa ANDRUZZI, Ben MCNALLY, Angela DELUCIA, Dona HEVRONI, Elizabeth CARPENTER, Magnus BESEV
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Patent number: 11421264Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: March 13, 2017Date of Patent: August 23, 2022Assignee: IsoPlexis CorporationInventors: Mong Sano Marma, Luisa Andruzzi, Ben McNally, Angela DeLucia, Dona Hevroni, Elizabeth Carpenter, Magnus Besev
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Publication number: 20220177859Abstract: The present invention relates to a polymerase enzyme with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 or any polymerase that shares at least 70% amino acid sequence identity thereto, comprising a mutation selected from the group of (i) at position 412 of SEQ ID NO. 1: serine (S) (L412S) and/or, (ii) at position 413 of SEQ ID NO. 1: glycine (G) (Y413G) and/or (iii) at position 414 of SEQ ID NO. 1: serine (S) (P414S), wherein the enzyme has little or no 3?-5? exonuclease activity.Type: ApplicationFiled: February 13, 2018Publication date: June 9, 2022Inventors: Jerzy Olejnik, Angela Delucia, Ralf Peist, Nicole Grasse
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Publication number: 20220145272Abstract: The present invention relates to a polymerase enzyme with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 with mutations in the amino acid sequence positions 409, 410 and 411.Type: ApplicationFiled: February 13, 2018Publication date: May 12, 2022Inventors: Jerzy Olejnik, Angela Delucia, Cheng-yao Chen, Ryan Charles Heller, Thomas William Schoenfeld
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Publication number: 20220127587Abstract: The present invention relates to a polymerase enzyme from Pyrococcus furiosus with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 or any polymerase that shares at least 70% amino acid sequence identity thereto, comprising a mutation selected from the group of (i) at position 409 of SEQ ID NO. 3: serine (S) (L409S) and/or, (ii) at position 410 of SEQ ID NO. 3: glycine (G) (Y410G) and/or (iii) at position 411 of SEQ ID NO. 3: serine (S) (P411S), wherein the enzyme has little or no 3?-5? exonuclease activity.Type: ApplicationFiled: February 13, 2018Publication date: April 28, 2022Inventors: Jerzy Olejnik, Angela Delucia, Cheng-yao Chen, Ryan Charles Heller, Thomas William Schoenfeld
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Publication number: 20210317423Abstract: The present invention relates to a polymerase enzyme from 9° N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.Type: ApplicationFiled: June 17, 2021Publication date: October 14, 2021Inventors: Jerzy Olejnik, Angela Delucia
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Publication number: 20210002720Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: August 21, 2020Publication date: January 7, 2021Inventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Patent number: 10844430Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: January 24, 2018Date of Patent: November 24, 2020Assignee: Qiagen Sciences, LLCInventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Publication number: 20200002689Abstract: The present invention relates to a polymerase enzyme from 9°N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.Type: ApplicationFiled: February 13, 2018Publication date: January 2, 2020Inventors: Jerzy Olejnik, Angela Delucia
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Publication number: 20190226018Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: January 24, 2018Publication date: July 25, 2019Inventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Publication number: 20180127819Abstract: The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis (SBS) methods. In particular, the present invention contemplates the use of chelators in washing reagents to improve SBS performance.Type: ApplicationFiled: October 31, 2017Publication date: May 10, 2018Inventors: Luisa Andruzzi, Ben McNally, Angela DeLucia, Dona Hevroni
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Publication number: 20170211134Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: March 13, 2017Publication date: July 27, 2017Inventors: Mong Sano Marma, Luisa Andruzzi, Ben MCNally, Angela DeLucia, Dona Hevroni, Elizabeth Carpenter, Magnus Besev