Abstract: The present invention is directed to use of azoxystrobin to decrease the number of flakes on a surface; the use of azoxystrobin to decrease itch on a surface as perceived by a user; the use of azoxystrobin to decrease the level of myeloperoxidase on a surface as an indicator of decrease of oxidative stress and damage. The present invention is directed to the use of azoxystrobin wherein the azoxystrobin is applied from a personal care composition such as a shampoo, conditioner or leave on treatment.

Abstract: One or more methods for sampling gingival metabolites and biomarkers.

Abstract: One or more methods for sampling gingival metabolites and biomarkers.

Abstract: A noninvasive method for diagnosing skin health in a subject comprising collecting a skin sample from the subject; detecting a level of one or more small molecule biomarkers in the epithelial cell sample/skin cell sample; diagnosing the subject as having a skin condition based on the level of a detected small molecule biomarker, wherein the detected small molecule is at least one compound chosen from: a compound generated by metabolism of amino acids, a compound generated by dipeptides metabolism, a compound generated by nucleic acids, a compound generated by metabolism of lipids, a compound generated by metabolism of carbohydrates, and mixtures thereof and further small molecule biomarkers as listed in Table 1. Further, a noninvasive method for evaluating the efficacy of products for skin health.

Abstract: A noninvasive method for diagnosing skin health in a subject comprising collecting a skin sample/epithelial cell sample from the subject; detecting a level of one or more biomarkers selected from the group consisting of Myeloperoxidase and oxidized lipids in the epithelial cell sample/skin cell sample; diagnosing the subject as having oxidative stress and/or oxidative damage based on the level of a detected biomarker. Further, a noninvasive method for evaluating the efficacy of products for skin health.

Abstract: A noninvasive method for measuring skin health in a subject comprising collecting an epithelial cell/skin cell sample from the subject; detecting a level of one or more antimicrobial peptide biomarkers in the epithelial cell sample/skin cell sample; diagnosing the subject as having a defense mechanism due to microbial attack on skin or a measure of the system being in balance with environment homeostasis based on the level of one or more antimicrobial peptide biomarkers selected from the group consisting of Histone H2B type 1-M, Histone H2A, Histone H4, Protein S100-A7, Protein S100-A8, Protein S100-A9, Cathepsin G, Neutrophil defensin 3 (Defensin, alpha 3) (HNP-3), Dermcidin, Ribonuclease 7 (RNase 7), human beta-defensin -2 (nBD-2) and Beta-defensin 103 (hBD-3) and listed in Table 1 wherein the AMP families include bactericidal permeability-increasing protein (BPI), Defensin, Histone, Pore-forming toxin, S100 protein, Cytotoxin, Serine Protease 51, Dual Oxidase, transcription regulation, and mixtures thereof

Abstract: A method for measuring of histamine in an epidermis comprising applying an adhesive article to an epithelium of a mammal; allowing for adherence of epithelial cells to the adhesive article; removing the adhesive article from the epithelium of the mammal; preparing the adhesive article using standard laboratory methods for extraction; extracting histamine from the epithelial cells adhered to said adhesive article; measuring histamine from the epithelial cells adhered to said adhesive article; determining the amount of histamine in the epithelial cells as compared to a baseline sample. Further, a method of objectively measuring the perception of itch in mammals and wherein there is a reduction in histamine from a baseline level which is directly proportional to a reduction in an itch perception.

Abstract: A method for measuring of histamine in an epidermis comprising applying an adhesive article to an epithelium of a mammal; allowing for adherence of epithelial cells to the adhesive article; removing the adhesive article from the epithelium of the mammal; preparing the adhesive article using standard laboratory methods for extraction; extracting histamine from the epithelial cells adhered to said adhesive article; measuring histamine from the epithelial cells adhered to said adhesive article; determining the amount of histamine in the epithelial cells as compared to a baseline sample. Further, a method of objectively measuring the perception of itch in mammals and wherein there is a reduction in histamine from a baseline level which is directly proportional to a reduction in an itch perception.