Abstract: A method for removing undesired component(s) from bound desired component(s) in an analysis including: (a) providing a container having a desired component bound thereto and an undesired component; (b) dispensing wash fluid in the container at a first level sufficient to contact a portion of the bound desired component; (c) oscillating wash fluid level in the container; and (d) removing a portion of the wash fluid from the container. A method for removing undesired components from bound desired components in an analysis including steps (a) and (b) above; (c) removing the wash fluid; (d) dispensing a wash fluid at a subsequent level lower than the first level and sufficient to wash the bound desired substrate; and (e) removing wash fluid. Preferably, the desired component is an analyte being measured.