Abstract: An article, process, and method for surface plasmon resonance plates are described. A substrate is covered with a thin metal film onto which a second thin metal film is deposited. The surface of the second thin metal film is converted to the metal oxide which is used to covalently bond organosilanes to the surface. Reactive organosilanes containing terminal bonding groups are arranged in a plurality of spots that are surrounded by inert organosilanes. Biomolecule attachment to the binding group is detected or measured from surface plasmon signals from the first thin metal film.

Abstract: A method for chemical analysis is provided comprising using a substrate that includes a hydrophilic silicon oxide surface and a hydrophobic self-assembled silane monolayer overlying and covalently bonded to portions of the silicon oxide surface to form a pattern defining a plurality of microwells, forming a solution comprising at least one chemical analyte in at least one of the microwells, and performing a chemical analysis on the solution.