Abstract: The present invention provides new coumarin-polymer conjugates that, upon irradiation with IR light, undergo an intermolecular [2+2] cycloaddition reaction through coumarin units through the coumarin components of different units of said coumarin-polymer conjugate thereby forming a crosslinked product, compositions/mixtures comprising said conjugates, products comprising the crosslinked product, and uses thereof. The new coumarin-polymer conjugates of the invention are particularly useful for 3D bioprinting.

Abstract: The present invention relates generally to methods and materials for use in the production of implants, particularly luminal tissue implants, where the implants are engineered by seeding of an acellular scaffold or matrix with muscle cell precursors and fibroblasts, for example injection seeding using particular ratios of cells. The present invention provides methods for producing tissue engineered constructs for implantation into a subject which can utilise novel seeding processes described herein for improved cell engraftment and differentiation. In addition, the invention describes methods for treating an individual by implantation of the engineered constructs or tissues of the invention.

Abstract: The present invention relates generally to methods and materials for use in the production of implants, particularly luminal tissue implants, where the implants are engineered by seeding of an acellular scaffold or matrix with muscle cell precursors and fibroblasts, for example injection seeding using particular ratios of cells. The present invention provides methods for producing tissue engineered constructs for implantation into a subject which can utilise novel seeding processes described herein for improved cell engraftment and differentiation. In addition, the invention describes methods for treating an individual by implantation of the engineered constructs or tissues of the invention.