Patents by Inventor Annaleen Vermeulen
Annaleen Vermeulen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9988625Abstract: The present invention is directed to collections of templates for molecules such as RNA, as well as templates, devices, kits and methods for generating molecules from these collections. Through the use of various embodiments of the present invention one may efficiently and effectively obtain selected RNA molecules such as siRNA, shRNA, miRNA mimics and inhibitors, lncRNA, antisense RNA, aptamers, ribozymes, and sgRNA and sets of those molecules.Type: GrantFiled: January 7, 2014Date of Patent: June 5, 2018Assignee: Dharmacon, Inc.Inventors: Devin Leake, Annaleen Vermeulen, Brady Culver, Abel Licon, Joshua A. Stahl
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Patent number: 9334495Abstract: Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.Type: GrantFiled: November 23, 2010Date of Patent: May 10, 2016Assignee: ELITECHGROUP B.V.Inventors: Anastasia Khvorova, Annaleen Vermeulen, Rob Kaiser, Jon Karpilow, Nicolaas M. J. Vermeulen, Walt Mahoney
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Publication number: 20150353927Abstract: The present invention is directed to collections of templates for molecules such as RNA, as well as templates, devices, kits and methods for generating molecules from these collections. Through the use of various embodiments of the present invention one may efficiently and effectively obtain selected RNA molecules such as siRNA, shRNA, miRNA mimics and inhibitors, lncRNA, antisense RNA, aptamers, ribozymes, and sgRNA and sets of those molecules.Type: ApplicationFiled: January 7, 2014Publication date: December 10, 2015Inventors: Devin Leake, Annaleen Vermeulen, Brady Culver, Abel Licon, Joshua A. Stahl
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Patent number: 8980855Abstract: Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.Type: GrantFiled: June 7, 2012Date of Patent: March 17, 2015Assignees: Elitech Holding B.V., GE Healthcare Dharmacon, Inc.Inventors: Anastasia Khvorova, Annaleen Vermeulen, Rob Kaiser, Jon Karpilow, Nicolaas M. J. Vermeulen, Walt Mahoney
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Patent number: 8415466Abstract: Disclosed are methods of enhancing functionality of duplex oligonucleotides and compositions made by the methods. The duplex oligonucleotides include siRNAs, miRNA mimics, and piRNA mimics which contain modified nucleotides and mismatches between the two strands of the molecule at specific nucleotide positions.Type: GrantFiled: April 30, 2012Date of Patent: April 9, 2013Assignee: Dharmacon, Inc.Inventors: Anastasia Khvorova, Devin Leake, Barbara Robertson, Annaleen Vermeulen, Christina Yamada
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Patent number: 8367318Abstract: The disclosure provides methods for inhibiting the activity of a miRNA cluster in a cell, and also for screening a cell for a phenotype(s) of interest resulting from inhibition of a miRNA cluster. The methods use a cluster pool which comprises at least one miRNA inhibitor specific for each miRNA in the miRNA cluster. MiRNA inhibitors are described that induce apoptosis in breast cancer cells and hence are useful in the treatment of breast cancer. The disclosure also provides pharmaceutical compositions which are useful for the treatment of breast cancer; methods for inducing the nuclear translocation of NF-?B in a breast cancer cell; methods for inducing the nuclear translocation of c-Jun in a breast cancer cell; method for inhibiting the nuclear translocation of NF-?B in a breast cancer cell; and methods for providing prognostic medical information relating to breast cancer progression.Type: GrantFiled: July 21, 2008Date of Patent: February 5, 2013Assignee: Dharmacon, Inc.Inventors: Anja Smith, Scott Baskerville, Devin Leake, Annaleen Vermeulen, Barbara Robertson, Anastasia Khvorova
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Publication number: 20120259001Abstract: Disclosed are methods of enhancing functionality of duplex oligonucleotides and compositions made by the methods. The duplex oligonucleotides include siRNAs, miRNA mimics, and piRNA mimics which contain modified nucleotides and mismatches between the two strands of the molecule at specific nucleotide positions.Type: ApplicationFiled: April 30, 2012Publication date: October 11, 2012Applicant: DHARMACON, INC.Inventors: Anastasia Khvorova, Devin Leake, Barbara Robertson, Annaleen Vermeulen, Christina Yamada
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Publication number: 20120245219Abstract: Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.Type: ApplicationFiled: June 7, 2012Publication date: September 27, 2012Applicants: DHARMACON, INC., ELITECH HOLDING B.V.Inventors: Anastasia Khvorova, Annaleen Vermeulen, Rob Kaiser, Jon Karpilow, Nicolaas M.J. Vermeulen, Walt Mahoney
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Patent number: 8188060Abstract: Disclosed are methods of enhancing functionality of duplex oligonucleotides and compositions made by the methods. The duplex oligonucleotides include siRNAs, miRNA mimics, and piRNA mimics which contain modified nucleotides and mismatches between the two strands of the molecule at specific nucleotide positions.Type: GrantFiled: February 10, 2009Date of Patent: May 29, 2012Assignee: Dharmacon, Inc.Inventors: Anastasia Khvorova, Devin Leake, Barbara Robertson, Annaleen Vermeulen, Christina Yamada
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Publication number: 20110172289Abstract: Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.Type: ApplicationFiled: November 23, 2010Publication date: July 14, 2011Inventors: Anastasia Khvorova, Annaleen Vermeulen, Rob Kaiser, Jon Karpilow, Nicolaas M.J. Vermeulen, Walt Mahoney
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Publication number: 20100273856Abstract: The disclosure provides methods for inhibiting the activity of a miRNA cluster in a cell, and also for screening a cell for a phenotype(s) of interest resulting from inhibition of a miRNA cluster. The methods use a cluster pool which comprises at least one miRNA inhibitor specific for each miRNA in the miRNA cluster. MiRNA inhibitors are described that induce apoptosis in breast cancer cells and hence are useful in the treatment of breast cancer. The disclosure also provides pharmaceutical compositions which are useful for the treatment of breast cancer; methods for inducing the nuclear translocation of NF-?B in a breast cancer cell; methods for inducing the nuclear translocation of c-Jun in a breast cancer cell; method for inhibiting the nuclear translocation of NF-?B in a breast cancer cell; and methods for providing prognostic medical information relating to breast cancer progression.Type: ApplicationFiled: July 21, 2008Publication date: October 28, 2010Applicant: DHARMACON INC.Inventors: Anja Smith, Scott Baskerville, Devin Leake, Annaleen Vermeulen, Barbara Robertson, Anastasia Khvorova
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Publication number: 20100184209Abstract: The present invention provides compositions and methods for inhibiting gene silencing by the RNAi pathway. The RNAi inhibitors of the invention have a reverse complement (RC) region to the target molecule of interest (e.g., miRNA) in association with at least one flanking region coupled to either at the 3? or 5? end of the RC region. The flanking regions can be single-stranded or can have one or more regions of double stranded nucleic acid with or without a hairpin loop. The RNAi inhibitors described herein can inhibit endogenous targets, including but not limited to microRNAs, or piRNAs, or can be used to inhibit the effects of exogenously introduced molecules, such as synthetic siRNAs, siRNAs expressed from vector constructs (e.g., viral expression systems), or siRNAs generated by enzymatic methods. Inhibition is specific, potent, prolonged, and can be performed on a single target or multiple targets simultaneously.Type: ApplicationFiled: February 16, 2007Publication date: July 22, 2010Applicant: Dharmacon, Inc.Inventors: Annaleen Vermeulen, Barbara Robertson, Scott Baskerville, Christina Yamada, Devin Leake, Yuriy Fedorov, Jon Karpilow, Anastasia Khvorova
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Publication number: 20090209626Abstract: Disclosed are methods of enhancing functionality of duplex oligonucleotides and compositions made by the methods. The duplex oligonucleotides include siRNAs, miRNA mimics, and piRNA mimics which contain modified nucleotides and mismatches between the two strands of the molecule at specific nucleotide positions.Type: ApplicationFiled: February 10, 2009Publication date: August 20, 2009Applicant: Dharmacon, Inc.Inventors: Anastasia Khvorova, Devin Leake, Barbara Robertson, Annaleen Vermeulen, Christina Yamada
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Publication number: 20060223777Abstract: The present invention provides improved hairpin and fractured hairpin constructs for use in gene silencing through the RNA interference pathway. An exemplary short hairpin polynucleotide for use in gene silencing can include a polynucleotide having from about 42 nucleotides to about 106 nucleotides configured for being processed by Dicer. The polynucleotide can include a first region having from about 19 to about 35 nucleotides, a loop region coupled to the first region, the loop region having from about 4 to about 30 nucleotides, and a second region having from about 19 to about 35 nucleotides and having at least about 80% complementarity to the first region. Optionally, one of the first region or second region can have an overhang having less than about 6 nucleotides. Also, the short hairpin can be formed of a plurality of polynucleotides that cooperate to form a hairpin structure.Type: ApplicationFiled: March 28, 2006Publication date: October 5, 2006Inventors: Annaleen Vermeulen, Angela Reynolds, Jon Karpilow, Devin Leake, Xiaoqin Cheng, Stephanie Hartsel, Anastasia Khvorova