Abstract: A process for preparing a biological sample including biological species, implemented in a preparation system, the preparation system including a device that includes: a housing, a first channel provided in the housing, a second channel provided in the housing, a chamber into which the first channel and the second channel open, a filter separating the chamber into two distinct spaces, the process including the following steps: injection of the biological sample in the form of a fluid via the first channel to concentrate biological species in the first space of the chamber of the device, injection of an immunological buffer fluid via the second channel of the device so as to at least partially elute the biological species with the immunological buffer fluid.

Abstract: A microfluidic device includes a single rigid support and a first unit having a first chamber of nonzero volume delimited by walls of the support. A filter separates the first chamber into a first space and a second space, a first channel made in the support and a second channel. The device also includes a second unit including a second chamber, a third channel. The device further includes a first fluidic transfer channel between the first chamber and the second chamber, made in the support and opening on the one hand into said second chamber and on the other hand at a first bypass node in the second channel. The device including first flow switching means arranged for selecting connection of the first chamber to the exterior only, via the second channel only or to the second chamber only, through the first transfer channel.

Abstract: An automated system for preparing a biological sample containing biological species, and including a support plate wherein one or more through-wells are made, each through-well having two opposite accesses, a first access and a second access, the first access being separated from the second access by a filter-forming porous wall against which the biological sample that is to undergo lysis may be placed, and, for each well: a first mobile member, that can be actuated to move translationally along the axis of the well so as to become inserted across the first access thereof, a second mobile member, that can be actuated to move translationally along the axis of the well so as to become inserted across the second access thereof, stimulation means of mechanical and/or thermal type for stimulating each first mobile member and/or each second mobile member and/or the support plate.

Abstract: A microfluidic system is intended for the analysis of a biological sample containing biological species. The system includes an optical detection device having a source configured to emit an optical signal and at least one sensor having a capture surface defining an optical signal reading zone. The system also includes a microfluidic device having a support in which an amplification chamber, in which an amplification reaction can be carried out, is made, and having an input channel opening into the amplification chamber. The amplification chamber includes at least one first zone located in the sensor reading zone and at least one protuberance forming a recess intended to receive a compound for internal control of the amplification reaction and arranged to be located outside the sensor reading zone or configured to be opaque to said optical signal.

Abstract: A device for preparing a blood sample, including a microfluidic card including: a first chamber for separating and/or extracting proteins to be analyzed that are present in the blood sample; a second chamber used for an operation involving digestion of proteins of different species that are present in the sample, to obtain a second sample containing digested peptides and nondigested proteins; and a third chamber connected to the second chamber to receive the second sample containing the digested peptides and the non-digested proteins, the third chamber being used for an operation involving purification and stabilization of the digested peptides.

Abstract: A method for detecting target biological species in a biological sample, the method being implemented in a device that makes it possible to concatenate the preparation of a sample for a detection via selective capture and a detection via biomolecular amplification.

Abstract: An automated system for preparing, detecting and analyzing a first fluid sample containing biological species, the preparation, detection and analysis system including at least one fluidic cartridge that includes at least one fluidic concentration and lysis module and one fluidic detection module including an array of several amplification chambers arranged in parallel, an apparatus including a mechanical assembly comprising at least one movable rod fastened to the frame and comprising a free end arranged to cooperate with a flexible membrane of the fluidic concentration and lysis module, an optical measurement system for measuring fluorescence through one or more amplification chambers of the fluidic detection module of the cartridge, a control and processing unit.

Abstract: The invention relates to a method for tracking the amplification of a sequence of nucleotides in a sample (10). The sample is placed between a light source (12) and an image sensor (16). Under the effect of amplification reagents, mixed with the sample, a nucleotide sequence, called the target sequence, is replicated iteratively, amplifying the target sequence. The method includes the acquisition of an image representative of the formation of a precipitate in the sample under the effect of the amplification, on the basis of which an image of interest is formed. The application of a statistical indicator to the image of interest allows an indicator of the amplification of the target sequence to be determined.

Abstract: A microfluidic system is intended for the analysis of a biological sample containing biological species. The system includes an optical detection device having a source configured to emit an optical signal and at least one sensor having a capture surface defining an optical signal reading zone. The system also includes a microfluidic device having a support in which an amplification chamber, in which an amplification reaction can be carried out, is made, and having an input channel opening into the amplification chamber. The amplification chamber includes at least one first zone located in the sensor reading zone and at least one protuberance forming a recess intended to receive a compound for internal control of the amplification reaction and arranged to be located outside the sensor reading zone or configured to be opaque to said optical signal.

Abstract: A microfluidic device includes a single rigid support and a first unit having a first chamber of nonzero volume delimited by walls of the support. A filter separates the first chamber into a first space and a second space, a first channel made in the support and a second channel. The device also includes a second unit including a second chamber, a third channel. The device further includes a first fluidic transfer channel between the first chamber and the second chamber, made in the support and opening on the one hand into said second chamber and on the other hand at a first bypass node in the second channel. The device including first flow switching means arranged for selecting connection of the first chamber to the exterior only, via the second channel only or to the second chamber only, through the first transfer channel.

Abstract: A device for analyzing a biological sample which includes a separation and detection chamber into which an injection channel and a discharge channel open, a filter separating the chamber into two distinct spaces so as to define a first space into which the injection channel opens and a second space into which the discharge channel opens, the filter having a porosity suitable for the separation to be carried out, a rough bearing surface having a surface roughness parameter suitable for carrying out a mechanical lysis of the biological species present in the sample, the bearing surface being arranged in the first space, a flexible membrane arranged opposite the rough bearing surface relative to the filter and blocking an opening made through the housing.

Abstract: A device for preparing a blood sample, including a microfluidic card including: a first chamber for separating and/or extracting proteins to be analyzed that are present in the blood sample; a second chamber used for an operation involving digestion of proteins of different species that are present in the sample, to obtain a second sample containing digested peptides and nondigested proteins; and a third chamber connected to the second chamber to receive the second sample containing the digested peptides and the non-digested proteins, the third chamber being used for an operation involving purification and stabilization of the digested peptides.

Abstract: A process for preparing a biological sample including biological species, implemented in a preparation system, the preparation system including a device that includes: a housing, a first channel provided in the housing, a second channel provided in the housing, a chamber into which the first channel and the second channel open, a filter separating the chamber into two distinct spaces, the process including the following steps: injection of the biological sample in the form of a fluid via the first channel to concentrate biological species in the first space of the chamber of the device, injection of an immunological buffer fluid via the second channel of the device so as to at least partially elute the biological species with the immunological buffer fluid.

Abstract: A device and method for lysing biological species present in a fluid includes implementing a rough surface against which the objects to be lysed are crushed by a shearing motion. A device for mechanically lysing biological species has a first and second wall mounted movable relative to each other, between an initial position in which the walls are separated from each other, and a lysing position in which the first wall presses against the second wall. The first and second walls also are mounted shearingly movable relative to each other in the lysing position. At least one of the first or second walls has a rough bearing surface against the other wall and has a mean surface roughness parameter Ra of between 0.2 ?m and 10 ?m, and preferably between 0.2 ?m and 3 ?m.

Abstract: The invention relates to a method for tracking the amplification of a sequence of nucleotides in a sample (10). The sample is placed between a light source (12) and an image sensor (16). Under the effect of amplification reagents, mixed with the sample, a nucleotide sequence, called the target sequence, is replicated iteratively, amplifying the target sequence. The method includes the acquisition of an image representative of the formation of a precipitate in the sample under the effect of the amplification, on the basis of which an image of interest is formed. The application of a statistical indicator to the image of interest allows an indicator of the amplification of the target sequence to be determined.

Abstract: A device for analyzing a biological sample which includes a separation and detection chamber into which an injection channel and a discharge channel open, a filter separating the chamber into two distinct spaces so as to define a first space into which the injection channel opens and a second space into which the discharge channel opens, the filter having a porosity suitable for the separation to be carried out, a rough bearing surface having a surface roughness parameter suitable for carrying out a mechanical lysis of the biological species present in the sample, the bearing surface being arranged in the first space, a flexible membrane arranged opposite the rough bearing surface relative to the filter and blocking an opening made through the housing.

Abstract: A device and method for lysing biological species present in a fluid includes implementing a rough surface against which the objects to be lysed are crushed by a shearing motion. A device for mechanically lysing biological species has a first and second wall mounted movable relative to each other, between an initial position in which the walls are separated from each other, and a lysing position in which the first wall presses against the second wall. The first and second walls also are mounted shearingly movable relative to each other in the lysing position. At least one of the first or second walls has a rough bearing surface against the other wall and has a mean surface roughness parameter Ra of between 0.2 ?m and 10 ?m, and preferably between 0.2 ?m and 3 ?m.