Patents by Inventor Annemarie H. Ralston

Annemarie H. Ralston has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for the production of pure virally inactivated butyrylcholinesterase
    Publication number: 20090093038
    Abstract: The present invention provides a method for purifying butyrylcholinesterase from various biological fluids. Biological fluids include, e.g., blood, blood fractions, plasma, and bioreactor broths, and other such mixtures containing butyrylcholinesterase. In one embodiment, the invention provides a method for the production of purified, virally inactivated butyrylcholinesterase by contacting a biological fluid containing butyrylcholinesterase with a cationic exchange chromatography material, with an affinity chromatography material, and treating the fluid with solvent detergent. The resulting purified butyrylcholinesterase can also be subjected to a pasteurization step, and formulated in a sodium chloride/sodium phosphate solution for storage or lyophilization.
    Type: Application
    Filed: March 13, 2008
    Publication date: April 9, 2009
    Applicant: American National Red Cross
    Inventors: Annemarie H. Ralston, Billy L. Kolen, David John Hammond
  • Method for the production of pure virally inactivated butyrylcholinesterase
    Patent number: 7365173
    Abstract: The present invention provides a method for purifying butyrylcholinesterase from various biological fluids. Biological fluids include, e.g., blood, blood fractions, plasma, and bioreactor broths, and other such mixtures containing butyrylcholinesterase. In one embodiment, the invention provides a method for the production of purified, virally inactivated butyrylcholinesterase by contacting a biological fluid containing butyrylcholinesterase with a cationic exchange chromatography material, with an affinity chromatography material, and treating the fluid with solvent detergent. The resulting purified butyrylcholinesterase can also be subjected to a pasteurization step, and formulated in a sodium chloride/sodium phosphate solution for storage or lyophilization.
    Type: Grant
    Filed: February 4, 2002
    Date of Patent: April 29, 2008
    Assignee: American National Red Cross
    Inventors: Annemarie H. Ralston, Billy L. Kolen, David John Hammond
  • Method for the production of pure virally inactivated butyrylcholinesterase
    Publication number: 20030148488
    Abstract: The present invention provides a method for purifying butyrylcholinesterase from various biological fluids. Biological fluids include, e.g., blood, blood fractions, plasma, and bioreactor broths, and other such mixtures containing butyrylcholinesterase. In one embodiment, the invention provides a method for the production of purified, virally inactivated butyrylcholinesterase by contacting a biological fluid containing butyrylcholinesterase with a cationic exchange chromatography material, with an affinity chromatography material, and treating the fluid with solvent detergent. The resulting purified butyrylcholinesterase can also be subjected to a pasteurization step, and formulated in a sodium chloride/sodium phosphate solution for storage or lyophilization.
    Type: Application
    Filed: February 4, 2002
    Publication date: August 7, 2003
    Inventors: Annemarie H. Ralston, Billy L. Kolen, David John Hammond
  • Activation of pure prothrombin to thrombin with about 30% to about 40% sodium citrate
    Patent number: 6245548
    Abstract: A method for the production of thrombin from pure prothrombin is provided by converting pure prothrombin by treating pure prothrombin with sodium citrate in the absence of additional coagulation factors. Preferrably, a solution is used containing about 30% to about 40% sodium citrate.
    Type: Grant
    Filed: March 9, 2000
    Date of Patent: June 12, 2001
    Assignee: American National Red Cross
    Inventors: Annemarie H. Ralston, William N. Drohan
  • Method for purification of alpha-1 proteinase inhibitor
    Patent number: 6093804
    Abstract: The methods of the present invention provide a simple means for separating active and inactive Alpha Proteinase Inhibitor (API). The methods further provide means for purifying API at high yield (>70%), and at levels of purity (>90%) and activity (>90%) not heretofore available. Moreover, the methods of the present invention are simple (i.e., two chromatographic steps) and efficient; and are thus especially suitable to large scale purification processes. These methods will contribute substantially to alleviating the unmet demand for API for therapeutic purposes.
    Type: Grant
    Filed: September 24, 1998
    Date of Patent: July 25, 2000
    Assignee: American National Red Cross
    Inventors: Annemarie H. Ralston, William H. Drohan