Abstract: Systems and methods of biochemical analysis are provided. A method of performing biochemical assays includes loading a first plurality of reservoirs on a first liquid carrier with a first plurality of compounds, coupling a second carrier with the first carrier, the second carrier having a second plurality of reservoirs configured to couple with the first plurality of reservoirs and containing a second plurality of compounds, transferring at least a portion of the first plurality of compounds to the second plurality of reservoirs, and separating at least one component from the second plurality of reservoirs. A system for biochemical analysis includes a first carrier and a second carrier. The first carrier includes a first substrate and a plurality of reservoirs in the substrate for retaining a first plurality of compounds.

Abstract: The invention provides methods and kits for detecting and/or quantifying nucleic acid sequences of interest by using pairs of probes containing donor-acceptor moieties that when hybridized on a target polynucleotide, with one of the probes being hybridized to a sequence of interest in the target polynucleotide, places the donor-acceptor moieties in sufficiently close proximity such that a detectable signal is generated. Methods of the invention are particularly useful for genotyping analysis and gene expression profiling. Methods of the invention are easily adaptable to arrays and automation. The invention also provides probes with enhanced hybridization efficiency and/or specificity comprising a probe portion having a spacer element and/or a minor groove binder molecule, and methods and kits for using these probes.

Abstract: A capillary bundle having reaction wells in one end of the capillaries is disclosed. The reaction wells serve as sites for hybridization, compound reaction, and drug identification for instance. The capillaries may be light-conducting capillaries. Also disclosed are various methods of identifying a target compound in a liquid carrier using this capillary bundle as well as methods of fabricating the bundle.