Abstract: The present invention is a simplified, highly-purified, processive translation system that does not require the addition of translation factors EF-P, W, W2 or rescue. A new translation process offers new, potentially improved, routes to all peptides, proteins and peptidomimetics currently synthesized by alternative routes.

Abstract: The present invention is a simplified, highly-purified, processive translation system that does not require the addition of translation factors EF-P, W, W2 or rescue. A new translation process offers new, potentially improved, routes to all peptides, proteins and peptidomimetics currently synthesized by alternative routes.

Abstract: It has been discovered that it is possible to target any RNA molecule for cleavage by RNase P by forming a hybrid region consisting of a short sequence of base pairs followed by a terminal 3'- NCCA sequence. In the preferred embodiment, the region is formed by addition of an external guide sequence consisting of a nucleotide sequence complementary to the targeted site which includes a 3'-NCCA, wherein the sequence hybridizes to the targeted RNA to form a short sequence of double-stranded RNA under conditions promoting cleavage of the substrate at the nucleotide at the 5' side of the base-paired region by the RNase P or catalytically active equivalent thereof. Specificity is determined by the complementary sequence. The sequence is preferably ten to fifteen nucleotides in length and may contain non-complementary nucleotides to the extent this does not interfere with formation of several base pairs followed by a NCCA at the 3' end.