Patents by Inventor Anthony R. Torres
Anthony R. Torres has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20170234893Abstract: The present invention provides methods for analyzing a target compound from a biological sample. In one aspect, a method for analyzing a target compound in a biological sample can comprise delivering a biological sample through an affinity column, the affinity column having a binding ligand coupled to a stationary structural support, wherein the affinity column has a high density of the binding ligand per the stationary structural support and wherein the binding ligand has been preselected to cause weak affinity separation zonal retardation of the target compound from the biological sample forming a target compound fraction and a biological sample fraction and detecting the target compound by mass spectrometry.Type: ApplicationFiled: September 19, 2016Publication date: August 17, 2017Inventors: Sten Ohlson, Anthony R. Torres
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Patent number: 9448243Abstract: The present invention provides methods for analyzing a target compound from a biological sample. In one aspect, a method for analyzing a target compound in a biological sample can comprise delivering a biological sample through an affinity column, the affinity column having a binding ligand coupled to a stationary structural support, wherein the affinity column has a high density of the binding ligand per the stationary structural support and wherein the binding ligand has been preselected to cause weak affinity separation zonal retardation of the target compound from the biological sample forming a target compound fraction and a biological sample fraction and detecting the target compound by mass spectrometry.Type: GrantFiled: September 4, 2014Date of Patent: September 20, 2016Inventors: Sten Ohlson, Anthony R. Torres
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Patent number: 9359637Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: GrantFiled: November 23, 2015Date of Patent: June 7, 2016Assignee: Great Basin Scientific, Inc.Inventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Publication number: 20160076080Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: ApplicationFiled: November 23, 2015Publication date: March 17, 2016Inventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Patent number: 9200312Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: GrantFiled: October 3, 2013Date of Patent: December 1, 2015Assignee: Great Basin Scientific CorporationInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Publication number: 20150125877Abstract: The present invention provides methods for analyzing a target compound from a biological sample. In one aspect, a method for analyzing a target compound in a biological sample can comprise delivering a biological sample through an affinity column, the affinity column having a binding ligand coupled to a stationary structural support, wherein the affinity column has a high density of the binding ligand per the stationary structural support and wherein the binding ligand has been preselected to cause weak affinity separation zonal retardation of the target compound from the biological sample forming a target compound fraction and a biological sample fraction and detecting the target compound by mass spectrometry.Type: ApplicationFiled: September 4, 2014Publication date: May 7, 2015Inventors: Sten Ohlson, Anthony R. Torres
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Patent number: 8993334Abstract: The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-though protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest.Type: GrantFiled: November 5, 2012Date of Patent: March 31, 2015Inventor: Anthony R. Torres
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Publication number: 20140038200Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: ApplicationFiled: October 3, 2013Publication date: February 6, 2014Applicant: Great Basin ScientificInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Patent number: 8574833Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: GrantFiled: November 26, 2008Date of Patent: November 5, 2013Assignee: Great Basin ScientificInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Publication number: 20130164827Abstract: The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-though protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest.Type: ApplicationFiled: November 5, 2012Publication date: June 27, 2013Inventor: Anthony R. Torres
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Patent number: 8304248Abstract: The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-through protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest.Type: GrantFiled: November 16, 2009Date of Patent: November 6, 2012Inventor: Anthony R. Torres
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Publication number: 20110118453Abstract: The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-though protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest.Type: ApplicationFiled: November 16, 2009Publication date: May 19, 2011Inventor: Anthony R. Torres
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Publication number: 20110039260Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: ApplicationFiled: November 26, 2008Publication date: February 17, 2011Applicant: Great Basin ScientificInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Publication number: 20090221096Abstract: Thin-film biosensor chips for detecting a target analyte in a biological sample are disclosed. The chips include a solid substrate, an antireflective optical layer, an attachment layer using a non-polymeric silane, and an Fc-specific binding molecule coupled to the non-polymeric silane. Kits containing the chips and methods of using and making the chips are also disclosed.Type: ApplicationFiled: December 13, 2006Publication date: September 3, 2009Applicant: Great Basin ScientificInventor: Anthony R. Torres
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Patent number: 6555661Abstract: High purity protein A is recovered from protein A preparations by passing a protein A preparation over an ion exchange column so that protein A will bind to a cation exchange resin. The loaded column is washed with a wash buffer solution having about the same pH as the protein A preparation and an increased mineral salt concentration to elute certain impurities but not the protein A. The protein A is eluted from the washed column by eluting the column with an elution buffer solution having a pH higher than the wash buffer solution and mineral salt concentration substantially no higher than the wash buffer solution. The protein A can be easily recovered from protein A preparations that do not use any human or animal components. The protein A purified by the invention is suitable for all common uses of protein A, but is especially well-suited for human therapeutic applications.Type: GrantFiled: February 25, 2000Date of Patent: April 29, 2003Inventors: Anthony R. Torres, Walter H. Runkis
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Patent number: 6551589Abstract: A method of reducing the chance of disease resulting from mononuclear cells in a body attacking desirable cell antigens in the body, such as antigens expressed by normal body cells or desirable engrafted cells, comprises reducing the number of mononuclear cell in the body that are likely to attack such desirable cell antigens. This is done by obtaining cell derived antigens subject to attack by mononuclear cells in the body and also obtaining mononuclear cells to be placed in the body. The mononuclear cells may be from a donor or may be removed from the subject body. The mononuclear cells are reacted, in vitro, with the cell derived antigens, such as by passing the mononuclear cells over a layer of fibroblast cells from the body expressing the desirable antigens, and recovering the mononuclear cells that do not bind to the antigens. These recovered cells are then infused into the body.Type: GrantFiled: April 28, 2000Date of Patent: April 22, 2003Inventor: Anthony R. Torres
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Patent number: 6056951Abstract: A method of reducing the chance of disease resulting from mononuclear cells in a body attacking desirable cell antigens in the body, such as antigens expressed by normal body cells or desirable engrafted cells, comprises reducing the number of mononuclear cell in the body that are likely to attack such desirable cell antigens. This is done by obtaining cell derived antigens subject to attack by mononuclear cells in the body and also obtaining mononuclear cells to be placed in the body. The mononuclear cells may be from a donor or may be removed from the subject body. The mononuclear cells are reacted, in vitro, with the cell derived antigens, such as by passing the mononuclear cells over a layer of fibroblast cells from the body expressing the desirable antigens, and recovering the mononuclear cells that do not bind to the antigens. These recovered cells are then infused into the body.Type: GrantFiled: October 7, 1997Date of Patent: May 2, 2000Inventor: Anthony R. Torres
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Patent number: 5028696Abstract: A method of separating charged molecules having different numbers of charges thereon includes the step of passing the molecules to be separated through ion-exchange material such as an ion-exchange chromatograpy column in normal manner so that molecules to be separated bind to the ion-exchange material. Displacer molecules are then passed through the ion-exchange column to selectively release certain of the molecules to be separated from the ion-exchange material, while others of the molecules to be separated remain bound to the material.Type: GrantFiled: October 28, 1988Date of Patent: July 2, 1991Inventors: Anthony R. Torres, Elbert A. Peterson