Abstract: An in vitro method is provided for analyzing chromatin accessibility and screening RNA of each single cell in a heterologous population (e.g., a library of cells). The method comprises incubating cell nuclei obtained from lysed cells with a transposome complex in a tagmentation buffer, performing reverse transcription wherein each of the RNAs is reverse transcribed to a DNA barcoded with the first barcode; sequencing DNA, which is extracted from digested cell nuclei; and analyzing chromatin accessibility and RNA of the cells. In a further embodiment, the method described comprises performing combinatorial cellular indexing and/or a perturbation step. Additionally, provided are a transposase TnY, buffer(s), and kit(s) for use in the described method.

Abstract: A packaging bag comprises a first ply and a second ply joined along an edge defining the closed end of the bag and along side edges of the bag. A mouth of the bag has a first edge on the first ply and a second edge on the second ply, which projects beyond the first edge and comprises a folded over film portion. The margin of the second edge portion is disposed adjacent the first edge so as to provide a substantially constant thickness of the bag across the mouth and projecting portion.