Abstract: The present invention refers to a method directed to RT-qPCR reactions, preferably performed in a one or two-step approach combining the reverse transcription and subsequent PCR in a single tube and buffer, using a reverse transcriptase along with a DNA polymerase with mandatory 3??5? exonuclease activity, which corrects miss-incorporated nucleotides. In particular, in this invention we present a One-step RT-PCR, preferably qPCR, method with a novel priming strategy that utilizes a novel bivalent reverse primer, wherein this primer is used for both, i) the generation of cDNA and ii) the completion of the subsequent amplification using that same cDNA as template. This bivalent reverse primer also allows end-tagging the amplicon(s) obtained so that they can be used in a variety of applications including, standard sequencing, next generation sequencing (NGS), gene expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing, and disease research.

Abstract: A method of determining the presence or absence of an antibody in a sample, the method comprising the steps of: (a) contacting the sample with a lysis composition comprising: (i) a quaternary ammonium compound; and (ii) a non-ionic surfactant; and (b) contacting the sample and the lysis composition with an antibody detection device.

Abstract: A nucleic acid detection method involving the direct generation of a measurable signal, by the action of an enzyme with 3?-5? nuclease activity, and the applications for it, the signal generated can be detectable and quantifiable and can be carried out in real-time, in this method, the nucleic acid is placed in contact with at least one oligonucleotide that does not hybridize perfectly with it, so that the enzyme will split it at the unpaired bases generating the signal, the oligonucleotide can be labeled.

Abstract: The invention relates to a method consisting of bringing into contact, in one container, (a) an aqueous solution of a reaction mixture comprising at least one enzyme, and (b) an aqueous solution of a stabilizing mixture comprising (i) at least one protective agent against drying, (ii) at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups, and (iii) at least one inert polymer capable of generating a mesh structure preventing the mobility of the dried reagents. The invention also consists of removing all or part of the water contained in the resulting aqueous solution. Said process is suitable for carrying out enzymatic reactions, for example, amplifying, sequencing and characterizing nucleic acids, performing hybridization tests and for the restriction analysis.

Abstract: A nucleic acid detection method involving the direct generation of a measurable signal, by the action of an enzyme with 3?-5? nuclease activity, and the applications for it, the signal generated can be detectable and quantifiable and can be carried out in real-time, in this method, the nucleic acid is placed in contact with at least one oligonucleotide that does not hybridize perfectly with it, so that the enzyme will split it at the unpaired bases generating the signal, the oligonucleotide can be labeled.

Abstract: The invention relates to a method consisting of bringing into contact, in one container, (a) an aqueous solution of a reaction mixture comprising at least one enzyme, and (b) an aqueous solution of a stabilizing mixture comprising (i) at least one protective agent against drying, (ii) at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups, and (iii) at least one inert polymer capable of generating a mesh structure preventing the mobility of the dried reagents. The invention also consists of removing all or part of the water contained in the resulting aqueous solution. Said process is suitable for carrying out enzymatic reactions, for example, amplifying, sequencing and characterizing nucleic acids, performing hybridization tests and for the restriction analysis.