Patents by Inventor Anurup GANGULI
Anurup GANGULI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11970730Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising and a blocking nucleic acid molecule represented by Formula III in the 5?-to-3? direction comprises: T-D-M-A-(B-L)J-C; wherein T is 17-135 nucleotides in length; D is 0-10 nucleotides in length; M is 1-25 nucleotides in length or is absent, wherein if M is absent then T-D and A-(B-L)J-C are separate nucleic acid strands; A is 0-15 nucleotides in length and comprises at least 50% sequence complementarity to D; B is 4-12 nucleotides in length and comprises at least 50% sequence complementarity to T; L is 3-25 nucleotides in length; J is an integer between 1 and 10; C is 4-15 nucleotides in length; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.Type: GrantFiled: June 10, 2023Date of Patent: April 30, 2024Assignee: VedaBio, Inc.Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir
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Patent number: 11965205Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more non-nucleic acid targets of interest in a sample. The compositions and methods provide signal boost upon detection of non-nucleic acid targets of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 25° C. or less, allow for massive multiplexing, high accuracy, minimal non-specific signal generation, and are easily reprogrammable.Type: GrantFiled: October 5, 2023Date of Patent: April 23, 2024Assignee: VedaBio, Inc.Inventors: Swetha Murali, Anurup Ganguli, Ketki Sawant, Ravi Vijayendran
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Publication number: 20240124919Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more non-nucleic acid targets of interest in a sample. The compositions and methods provide signal boost upon detection of non-nucleic acid targets of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 25° C. or less, allow for massive multiplexing, high accuracy, minimal non-specific signal generation, and are easily reprogrammable.Type: ApplicationFiled: October 5, 2023Publication date: April 18, 2024Inventors: Swetha Murali, Anurup Ganguli, Ketki Sawant, Ravi Vijayendran
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Publication number: 20240110251Abstract: Described herein are systems and methods which utilize an array of wells to rapidly detect target analytes from whole blood samples, even at very low concentrations. The provided systems and methods dry the blood sample to ensure a fluidic network forms in one or more dry blood sample islands and liquid with reagents useful for detecting nucleic acid sequences from a target analyte, including by amplification reactions that are bi-phasic because of the interaction between the solid dried blood sample phase and the liquid phases, including liquid having reagents that are able to fluidically and diffusively exchange with the fluidic network within the dried blood sample phase.Type: ApplicationFiled: September 25, 2023Publication date: April 4, 2024Inventors: Rashid BASHIR, Anurup Ganguli, Jongwon Lim, Ariana Mostafa, Enrique Andres Valera Cano, Matthew Wester, Jacob Berger
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Publication number: 20240110164Abstract: The present disclosure relates to compositions of matter and methods used to activate effector nucleic acids and effector targets in vivo via a CRISPR-based cascade system. The compositions and methods achieve non-specific delivery of cascade system components to cells yet the cascade system works in a cell-specific manner.Type: ApplicationFiled: September 29, 2023Publication date: April 4, 2024Inventors: Anurup Ganguli, Joshua Miller, Andrew Garst, Robert Plasschaert, Swetha Murali
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Patent number: 11946052Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.Type: GrantFiled: November 24, 2023Date of Patent: April 2, 2024Assignee: VedaBio, Inc.Inventors: Ariana Mostafa, Jacob Berger, Ashish Pandey, Anurup Ganguli
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Publication number: 20240102014Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.Type: ApplicationFiled: November 24, 2023Publication date: March 28, 2024Inventors: Ariana Mostafa, Jacob Berger, Ashish Pandey, Anurup Ganguli
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Publication number: 20240068053Abstract: The current disclosure provides a simple single-step room temperature Activate Cleave and Count (ACC) assay coupled to Photonic Resonator Absorption Microscopy (PRAM) in an amplification-free approach. The assay, and associated system and method disclosed herein allow for detection of viral and bacterial pathogens as well disease such as cancer at the point of care.Type: ApplicationFiled: December 31, 2021Publication date: February 29, 2024Inventors: Brian T. CUNNINGHAM, Anurup GANGULI, Taylor D. CANADY, Nantao LI, Ying XIONG, Shreya GHOSH, Yanyu XIONG, Lucas D. AKIN
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Publication number: 20240035028Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods provide signal boost upon detection of target nucleic acids of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 16° C. or less, without amplification of the target nucleic acids yet allowing for massive multiplexing, high accuracy and minimal non-specific signal generation.Type: ApplicationFiled: August 16, 2023Publication date: February 1, 2024Inventors: Anurup Ganguli, Ashish Pandey, Ariana Mostafa, Jacob Berger
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Patent number: 11884921Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods provide signal boost upon detection of target nucleic acids of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 16° C. or less, without amplification of the target nucleic acids yet allowing for massive multiplexing, high accuracy and minimal non-specific signal generation.Type: GrantFiled: December 9, 2022Date of Patent: January 30, 2024Assignee: VedaBio, Inc.Inventors: Anurup Ganguli, Ashish Pandey, Ariana Mostafa, Jacob Berger
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Patent number: 11884922Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.Type: GrantFiled: June 10, 2023Date of Patent: January 30, 2024Assignee: VedaBio, Inc.Inventors: Ariana Mostafa, Jacob Berger, Ashish Pandey, Anurup Ganguli
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Publication number: 20240026425Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising and a blocking nucleic acid molecule represented by Formula IV, wherein Formula IV in the 5?-to-3? direction comprises: T-D-M-A-Lp-C; wherein T is 17-31 nucleotides in length; D is 0-15 nucleotides in length; M is 1-25 nucleotides in length; A is 0-15 nucleotides in length and comprises a sequence complementary to D; and L is 3-25 nucleotides in length; p is 0 or 1; C is 4-15 nucleotides in length and comprises a sequence complementary to T; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.Type: ApplicationFiled: September 24, 2023Publication date: January 25, 2024Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir
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Publication number: 20240018518Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.Type: ApplicationFiled: June 10, 2023Publication date: January 18, 2024Inventors: Ariana Mostafa, Jacob Berger, Ashish Pandey, Anurup Ganguli
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Patent number: 11859182Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.Type: GrantFiled: May 31, 2023Date of Patent: January 2, 2024Assignee: VedaBio, Inc.Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey
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Patent number: 11845084Abstract: Provided are methods and related devices for preparing a cell and tissue culture, including a hanging drop culture. Microwells are specially loaded with cell mixtures using a removable reservoir and forcing cells into the underlying microwells. The removable reservoir is removed and the cells partitioned into the individual microwells and covered by an immiscible layer of fluid. The microwells and immiscible layer is inverted and the cells in the microwells cultured. The microwells may have shape-controlling elements to control the three-dimensional shape of the culture.Type: GrantFiled: May 4, 2021Date of Patent: December 19, 2023Assignees: The Board of Trustees of the University of Illinois, Mayo Foundation for Medical Education and ResearchInventors: Anurup Ganguli, Rashid Bashir, Panagiotis Z. Anastasiadis, George Vasmatzis
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Publication number: 20230399682Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising and a blocking nucleic acid molecule represented by Formula III in the 5?-to-3? direction comprises: T-D-M-A-(B-L)J-C; wherein T is 17-135 nucleotides in length; D is 0-10 nucleotides in length; M is 1-25 nucleotides in length or is absent, wherein if M is absent then T-D and A-(B-L)J-C are separate nucleic acid strands; A is 0-15 nucleotides in length and comprises at least 50% sequence complementarity to D; B is 4-12 nucleotides in length and comprises at least 50% sequence complementarity to T; L is 3-25 nucleotides in length; J is an integer between 1 and 10; C is 4-15 nucleotides in length; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.Type: ApplicationFiled: June 10, 2023Publication date: December 14, 2023Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir
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Patent number: 11820983Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.Type: GrantFiled: December 6, 2022Date of Patent: November 21, 2023Assignee: LabSimply, Inc.Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey
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Patent number: 11821025Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising and a blocking nucleic acid molecule represented by Formula I, wherein Formula I in the 5?-to-3? direction comprises: A-(B-L)J-C-M-T-D; wherein A is 0-15 nucleotides in length; B is 4-12 nucleotides in length; L is 3-25 nucleotides in length; J is an integer between 1 and 10; C is 4-15 nucleotides in length; M is 1-25 nucleotides in length or is absent, wherein if M is absent then A-(B-L)J-C and T-D are separate nucleic acid strands; T is 17-135 nucleotides in length and comprises at least 50% sequence complementarity to B and C; D is 0-10 nucleotides in length and comprises at least 50% sequence complementarity to A; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.Type: GrantFiled: May 31, 2023Date of Patent: November 21, 2023Assignees: VedaBio, Inc., The Board of Trustees of The University of IllinoisInventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir
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Publication number: 20230340573Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising and a blocking nucleic acid molecule represented by Formula I, wherein Formula I in the 5?-to-3? direction comprises: A-(B-L)J-C-M-T-D; wherein A is 0-15 nucleotides in length; B is 4-12 nucleotides in length; L is 3-25 nucleotides in length; J is an integer between 1 and 10; C is 4-15 nucleotides in length; M is 1-25 nucleotides in length or is absent, wherein if M is absent then A-(B-L)J-C and T-D are separate nucleic acid strands; T is 17-135 nucleotides in length and comprises at least 50% sequence complementarity to B and C; D is 0-10 nucleotides in length and comprises at least 50% sequence complementarity to A; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.Type: ApplicationFiled: May 31, 2023Publication date: October 26, 2023Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey, Rashid Bashir
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Publication number: 20230287423Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.Type: ApplicationFiled: May 31, 2023Publication date: September 14, 2023Inventors: Anurup Ganguli, Ariana Mostafa, Jacob Berger, Ashish Pandey