Abstract: A method and collection and transport device for collecting biological samples, such as a blood sample, from a subject wherein the method and device include a body having a slide member mounted therein and movable from a first position wherein a collection well of the slide member is aligned to receive a sample from the subject to a second position wherein the sample communicates with a chemical reagent contained within a cavity of the body. The sample well and chemical reagent cavity are sealed from the exterior of the body when the slide member is in the second position. In preferred embodiments, locking means are provided to retain the slide member in the second position. In some embodiments, a lancet mechanism may be provided with the transport and collection device.