Abstract: An apparatus 20 for the separation of a subpopulation of cells from an intact organ or other biological material is provided. The apparatus 20 includes: (1) a digestion chamber 24 that integrates the primary digestion process, (2) a measuring cylinder 26, (3) a cell collection chamber 28, (4) a heat exchanger 30 for raising and lowering temperatures in the digestion chamber 24 to activate or inactivate enzymes, (5) sensors 112, 114, 116, 118, 120, 122 to complete a closed feedback loop for allowing optimization of the digestion process, and (6) mock cells which mimic the cells to be harvested and which are used to fully optimize the process without unnecessary destruction of harvested cells. The manipulation of the digestion process may be manual or may be automated under computer control.

Abstract: Extra- to intra-corporeal power is provided by a transformer implanted at least partially within a defunctionalized intestinal pouch (or sack), such as an ileal pouch. The transformer includes a continuous loop magnetic core which is implanted within the pouch. The pouch itself includes a passageway permitting the secondary wiring to extend around the and through the magnetic core and through its central opening without entering the pouch providing intracorporeal current. Wire providing the primary windings extend from outside the body in through a stoma into the pouch and surround portions of the magnetic core within the pouch. Because of the use of a generally continuous loop magnetic core of high permeability, there is little or virtually no magnetic flux leakage. A solid circular core of a high permeability material may be used.