Patents by Inventor Asa Hagner-McWhirter
Asa Hagner-McWhirter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11513121Abstract: The present invention relates to a method for virus assay. More closely the invention relates a method for total quantification of adenovirus in a sample as well as total and functional (active) adenovirus in a sample. The method for determining adenovirus concentration in a sample comprises subjecting said sample to SPR (surface plasmon resonance) assay with immobilized FX (Factor X) and/or immobilized CAR (coxsackievirus and adenovirus receptor) on a sensor surface, wherein the adenovirus concentration is determined from sample binding to immobilized FX and/or immobilized CAR. CAR can be replaced by an ligand binding to adenovirus fiber, such as an anti-adenovirus fiber antibody. FX can be replaced by a ligand binding to adenovirus hexon, such as an anti-adenovirus hexon antibody. The method can be used for quality control in an adenovirus purification process, for example for gene therapy.Type: GrantFiled: November 8, 2018Date of Patent: November 29, 2022Assignee: CYTIVA SWEDEN ABInventors: Asa Frostell, Elisabeth Wallby, Asa Hagner-McWhirter, Linnea Nygren Babol
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Publication number: 20210292788Abstract: The present invention relates to a method for virus purification. The present invention provides downstream processes for purification of adenovirus from cell culture harvest. More closely, it relates to a method for adenovirus purification using a virus capture and a virus polishing step.Type: ApplicationFiled: April 24, 2019Publication date: September 23, 2021Inventors: Asa Hagner McWhirter, Anna Akerblom
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Publication number: 20200264175Abstract: The present invention relates to a method for virus assay. More closely the invention relates a method for total quantification of adenovirus in a sample as well as total and functional (active) adenovirus in a sample. The method for determining adenovirus concentration in a sample comprises subjecting said sample to SPR (surface plasmon resonance) assay with immobilized FX (Factor X) and/or immobilized CAR (coxsackievirus and adenovirus receptor) on a sensor surface, wherein the adenovirus concentration is determined from sample binding to immobilized FX and/or immobilized CAR. CAR can be replaced by an ligand binding to adenovirus fiber, such as an anti-adenovirus fiber antibody. FX can be replaced by a ligand binding to adenovirus hexon, such as an anti-adenovirus hexon antibody. The method can be used for quality control in an adenovirus purification process, for example for gene therapy.Type: ApplicationFiled: November 8, 2018Publication date: August 20, 2020Inventors: Asa Frostell, Elisabeth Wallby, Asa Hagner-McWhirter, Linnea Nygren Babol
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Patent number: 10732176Abstract: The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other. In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished.Type: GrantFiled: June 27, 2012Date of Patent: August 4, 2020Assignee: GE Healthcare Bio-Sciences ABInventors: Lennart Bjorkesten, Sofia Edlund, Asa Hagner-McWhirter
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Patent number: 8859215Abstract: The present invention relates to a fluorescent cell binding assay combining pre-labeling and Western blotting. Intact cells are incubated with pre-labelled binders preferably followed by SDS PAGE (sodium dodecylsulphate polyacrylamide gel) separation and Western blotting. More closely, the invention relates to a cell binding assay in which the degree or amount of binding of one or more cell interacting protein or protein component to the cell surface is measured with the ability to correlate the degree of cell binding to the sample load/total number of cells.Type: GrantFiled: June 27, 2012Date of Patent: October 14, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Susanne Grimsby, Asa Hagner-McWhirter
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Publication number: 20140141453Abstract: The present invention relates to a fluorescent cell binding assay combining pre-labeling and Western blotting. Intact cells are incubated with pre-labelled binders preferably followed by SDS PAGE (sodium dodecylsulphate polyacrylamide gel) separation and Western blotting. More closely, the invention relates to a cell binding assay in which the degree or amount of binding of one or more cell interacting protein or protein component to the cell surface is measured with the ability to correlate the degree of cell binding to the sample load/total number of cells.Type: ApplicationFiled: June 27, 2012Publication date: May 22, 2014Applicant: GE Healthcare Bio-Sciences ABInventors: Susanne Grimsby, Asa Hagner-McWhirter
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Publication number: 20140134752Abstract: The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other. In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished.Type: ApplicationFiled: June 27, 2012Publication date: May 15, 2014Applicant: GE Healthcare Bio-Sciences ABInventors: Lennart Bjorkesten, Sofia Edlund, Asa Hagner-McWhirter
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Publication number: 20140106373Abstract: The present invention relates to a method for target protein normalization, especially for Western blotting applications. More closely, the invention relates to a method for normalizing target protein signals, after electrophoresis and Western blotting, against variations of sample load or cell number between different lanes or within the same lane on an electrophoretic gel. The signals are normalized against the total protein signal (=ratio between target protein/total protein) or reference protein band signal(s) (=ratio between target protein/reference protein band). According to the invention multiplex and quantitative assessments are possible, such as quantitative comparison between target proteins in different samples.Type: ApplicationFiled: May 29, 2012Publication date: April 17, 2014Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Asa Hagner-Mcwhirter, Ola Ronn
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Publication number: 20130323729Abstract: The invention provides a method for detecting a biomolecular feature (a protein, protein complex, or modified protein such as a phosphorylated protein) by a modified Western blot type of assay, which method either electrophoretic gel separation followed by transfer, or direct spotting of a sample containing the biomolecular feature onto a membrane; providing a proximity probe pair, each probe comprising a binding moiety with affinity for a different binding site on the bio molecular feature and a reactive oligonucleotide, coupled thereto; binding the proximity probes to their respective binding sites on the biomolecular feature through the binding moiety, adding a splint oligonucleotide and a backbone oligonucleotide which are complementary to the reactive oligonucleotide pair, and allowing hybridization among them; ligating the hybridized DNA oligonucleotides to create a circularized DNA molecule when both probes bind sufficiently close to each other on the bio molecular feature, amplifying the circularizedType: ApplicationFiled: October 27, 2011Publication date: December 5, 2013Applicant: OLINK ABInventors: Ulf Landegren, Åsa Hagner-McWhirter, Daniel Ivansson
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Publication number: 20080314750Abstract: The present invention relates to a method for pre-fractionation of complex protein and/or peptide samples. The method comprises the following steps: a) loading the sample onto pre-packed media able to separate proteins/peptides according to their pl-value; b) eluting the media by centrifugal force under denaturing conditions with at least three buffers having different pH in a stepwise manner to obtain at least three fractions separated according to pl-value; and c) subjecting each fraction to further separation in a pH-range corresponding to the pl-values of said fractions. The invention also relates to a kit comprising at least one spin column or multiwell plate filled with chromatography medium able to separate proteins/peptides according to pl-value, at least three buffers, and one or more IPG strips having a narrow pH-range adapted to each of said buffers.Type: ApplicationFiled: November 14, 2006Publication date: December 25, 2008Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Asa Hagner-McWhirter, Jesper Hedberg