Abstract: Embryogenic cotton suspension cultures can be transformed by elongated, needle-like structures called “whiskers”. The process comprises the agitation of cotton suspension cultures in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated.

Abstract: Embryogenic cotton suspension cultures can be transformed by elongated, needle-like structures called “whiskers”. The process comprises the agitation of cotton suspension cultures in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated.

Abstract: Embryogenic cotton suspension cultures can be transformed by elongated, needle-like structures called “whiskers”. The process comprises the agitation of cotton suspension cultures in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated.

Abstract: A method is provided for asexually propagating pine trees, preferably loblolly pine, by vegatative propagation. According to this method, pine seedlings are hedged by severing the main stem and detaching the branches so that only one lateral branch is left intact and attached to the remaining main stem. The hedged seedlings are maintained under conventional growth conditions at least until elongated shoots having juvenile characteristics are obtained at the cut end of the main stem. The elongated shoots are then severed from the main stem and rooted by conventional means.

Abstract: A method of in vitro clonal propagation of plantlets from excised gymnosperm tissue in which the excised tissue is pulse treated on a nutrient medium containing at least about 10 mg/L, preferably at least about 20 mg/L, of a cytokinin for a time sufficient to induce formation of adventitious buds on the excised tissue and is then transferred to a nutrient medium free of exogenous growth factors and maintained thereon until the induced adventitious buds produce rootable shoots. The rootable shoots may then be rooted by conventional means but preferably are pulse treated on a nutrient medium containing a phenolic compound, preferably in an amount of at least about 5 mg/L, and an auxin, preferably in an amount of at least about 5 mg/L, for a time sufficient to induce formation of adventitious roots and then transferred to a nutrient medium free of exogenous growth factors until the shoots are rooted.

Abstract: A method for the in situ activation of the normally dormant needle fascicles of gymnosperms and the clonal propagation of gymnosperms and the clones produced thereby. The method for the in situ activation of the needle fascicles of gymnosperms to produce shoots comprising the step of contacting the terminal portion of a stem of the gymnosperm with between about 0.01 to 20 mg of a cytokinin. And, the method for the clonal propagation of gymnosperms and the production of clones thereof comprising the steps of contacting the terminal portion of a stem of the gymnosperm with between about 0.01 to 20 mg of a cytokinin; allowing the shoots to elongate; excising the elongated shoots from the stem and rooting the excised shoots.