Abstract: Macroporous beads and a method of manufacturing and using such macroporous beads. wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.

Abstract: Particle populations and assays are provided which have varying amounts of magnetic material and varying particle concentrations among different subsets of particles. In particular, the particle populations and assays include at least two particle sets with distinct sums of particles which are inversely related to the magnetic material concentration comprising each of the particles within the two particle sets, respectively. A method for processing an assay having such particle sets includes routing the assay in proximity to an imaging plane within a static imaging optical analysis system and generating a magnetic field in proximity to the imaging plane. The magnetic field is sufficient to attract and immobilize a ratio of particles from the at least two discrete particle sets that is different from the ratio of particles comprising the at least two discrete particle sets in the assay as the assay is introduced into the static imaging optical analysis system.

Abstract: Macroporous beads and a method of manufacturing and using such macroporous beads. wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.

Abstract: Macroporous beads and a method of manufacturing and using such macroporous beads. wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.

Abstract: Particle populations and assays are provided which have varying amounts of magnetic material and varying particle concentrations among different subsets of particles. In particular, the particle populations and assays include at least two particle sets with distinct sums of particles which are inversely related to the magnetic material concentration comprising each of the particles within the two particle sets, respectively. A method for processing an assay having such particle sets includes routing the assay in proximity to an imaging plane within a static imaging optical analysis system and generating a magnetic field in proximity to the imaging plane. The magnetic field is sufficient to attract and immobilize a ratio of particles from the at least two discrete particle sets that is different from the ratio of particles comprising the at least two discrete particle sets in the assay as the assay is introduced into the static imaging optical analysis system.

Abstract: Macroporous beads and a method of manufacturing and using such macroporous beads. wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.