Abstract: An acoustophoresis device includes an acoustic chamber with a piezoelectric element located within its volume. The piezoelectric element vibrates and generates acoustic standing waves from both sides, so that particles can be separated from fluid passing through the acoustic chamber. This permits the element to be cooled more efficiently, reducing transient heat loads in the fluid traveling through the device.

Abstract: Devices for separating a host fluid from a second fluid or particulate are disclosed. The devices include an acoustic chamber, a fluid outlet at a top end of the acoustic chamber, a concentrate outlet at a bottom end of the acoustic chamber, and an inlet on a first side end of the acoustic chamber. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the acoustic chamber that traps and separates particulates (e.g. cells) from a host fluid. The host fluid is collected via the fluid outlet, and the particulates are collected via the concentrate outlet. The device is a large-scale device that is able to process liters/hour, and has a large interior volume.

Abstract: Devices for separating materials from a host fluid are disclosed. The devices include a flow chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer and reflector create an angled acoustic standing wave oriented at an angle relative to the direction of mean flow through the flow chamber. The angled acoustic standing wave results in an acoustic radiation force having an axial force component that deflects the materials, so that the materials and the host fluid can thus be separated. The angled acoustic standing wave can be oriented at an angle of about 20° to about 70° relative to the direction of mean flow through the flow chamber to deflect, collect, differentiate, or fractionate the materials from the fluid flowing through the device at flow rates of about 400 mL/min up to about 700 mL/min.

Abstract: A baggage restraint system for the containment of luggage and other personal or group articles, such as camping gear or sport equipment, to the confines of two or more successive seats in a commercial passenger vehicle, such as a school bus. The system comprises an enclosure, and the means by which it may be releasably secured to the vehicle cabin floor. The system's enclosure is constructed of tear-resistant fabric panels, as an essentially rectangular or forward-leaning, four-sided cuboid. The enclosure is shaped and dimensioned to envelope a predetermined number of seats in a target vehicle. The system's means of securement include: a plurality of depending loops, affixed along one or more of the enclosure's bottom and windows-side edges; a plurality of floor and sidewall anchor tabs; and a plurality of carabiners and adjustable straps, linking each anchor tab to one of the depending loops.

Abstract: Data files are supplied to a plurality of requesting stations (102 to 106) by accessing serving devices (109 to 116). A plurality of requests are received for one or more data files at a traffic management system (101) from requesting stations via an external network (107). A specific server is selected at the traffic management system and a request is issued to the selected server over a local network for the requested file. The requested file is accepted at the traffic management system from the selected server over the local network and the requested file is sent from the traffic management system to the requesting station over external network. The response of selected servers is monitored (302) when responding to issued requests thereby generating monitored responses. The monitored responses are compared (303) against an operational criterion to identify sub-standard operations.

Abstract: The invention relates generally to polypeptides, such as antibody molecules, that demonstrate high stability and solubility. In particular, the invention relates to polypeptides comprising paired VL and VH domains that demonstrate soluble expression and folding in a reducing or intracellular environment. The invention also relates to polynucleotides encoding such polypeptides, to libraries of such polypeptides or polynucleotides, and to methods of using such polypeptides in research, diagnostic and therapeutic applications.

Abstract: Devices for separating a host fluid from a second fluid or particulate are disclosed. The devices include an acoustic chamber, a fluid outlet at a top end of the acoustic chamber, a concentrate outlet at a bottom end of the acoustic chamber, and an inlet on a first side end of the acoustic chamber. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the acoustic chamber that traps and separates particulates (e.g. cells) from a host fluid. The host fluid is collected via the fluid outlet, and the particulates are collected via the concentrate outlet. The device is a large-scale device that is able to process liters/hour, and has a large interior volume.

Abstract: An acoustophoresis device includes an acoustic chamber with a piezoelectric element located within its volume. The piezoelectric element vibrates and generates acoustic standing waves from both sides, so that particles can be separated from fluid passing through the acoustic chamber. This permits the element to be cooled more efficiently, reducing transient heat loads in the fluid traveling through the device.

Abstract: An acoustophoresis device which includes a substantially vertical flow path of the fluid mixture in order to improve separation of particles/secondary fluid from a primary fluid is disclosed. The vertical flow path reduces velocity non-uniformities in the acoustic chamber resulting from gravity forces. The device includes an acoustic chamber in which multidimensional acoustic standing waves are generated. The fluid can be introduced into the acoustic chamber using a dump diffuser in which a plurality of inlets enter near the bottom of the acoustic chamber such that flow symmetry reduces both, gravity driven flow non-uniformities, and any flow interference effects between inlet mixture flow into the acoustic chamber and the continuous gravity driven particle cluster drop out.

Abstract: Devices for separating materials from a host fluid are disclosed. The devices include a flow chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer and reflector create an angled acoustic standing wave oriented at an angle relative to the direction of mean flow through the flow chamber. The angled acoustic standing wave results in an acoustic radiation force having an axial force component that deflects the materials, so that the materials and the host fluid can thus be separated. The angled acoustic standing wave can be oriented at an angle of about 20° to about 70° relative to the direction of mean flow through the flow chamber to deflect, collect, differentiate, or fractionate the materials from the fluid flowing through the device at flow rates of about 400 mL/min up to about 700 mL/min.

Abstract: Methods, systems, and apparatus for acoustic separation of cellular supporting materials such as microcarriers or microbubbles from cell culture are provided.

Abstract: Devices for separating a host fluid from a second fluid or particulate are disclosed. The devices include an acoustic chamber, a fluid outlet at a top end of the acoustic chamber, a concentrate outlet at a bottom end of the acoustic chamber, and an inlet on a first side end of the acoustic chamber. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the acoustic chamber that traps and separates particulates (e.g. cells) from a host fluid. The host fluid is collected via the fluid outlet, and the particulates are collected via the concentrate outlet. The device is a large-scale device that is able to process liters/hour, and has a large interior volume.

Abstract: An acoustophoresis device includes an acoustic chamber with a piezoelectric element located within its volume. The piezoelectric element vibrates and generates acoustic standing waves from both sides, so that particles can be separated from fluid passing through the acoustic chamber. This permits the element to be cooled more efficiently, reducing transient heat loads in the fluid traveling through the device.

Abstract: Devices for separating materials from a host fluid are disclosed. The devices include a flow chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer and reflector create an angled acoustic standing wave oriented at an angle relative to the direction of mean flow through the flow chamber. The angled acoustic standing wave results in an acoustic radiation force having an axial force component that deflects the materials, so that the materials and the host fluid can thus be separated. The angled acoustic standing wave can be oriented at an angle of about 20° to about 70° relative to the direction of mean flow through the flow chamber to deflect, collect, differentiate, or fractionate the materials from the fluid flowing through the device at flow rates of about 400 mL/min up to about 700 mL/min.

Abstract: Methods, systems, and apparatus for acoustic separation of cellular supporting materials such as microcarriers or microbubbles from cell culture are provided.

Abstract: An apparatus includes a flow chamber having at least one inlet and at least one outlet. At least one ultrasonic transducer is located on a wall of the flow chamber, which operates to create a multi-dimensional acoustic standing wave in the flow chamber. A reflector is located on the wall on the opposite side of the flow chamber from the at least one ultrasonic transducer. The reflector is formed from a thin structure that provides a pressure release boundary, such as a plastic film/air interface.

Abstract: An acoustophoresis device made up of modular components is disclosed. Several modules are disclosed herein, including ultrasonic transducer modules, input/output modules, collection well modules, and various connector modules. These permit different systems to be constructed that have appropriate fluid dynamics for separation of particles, such as biological cells, from a fluid.

Abstract: Devices for separating a host fluid from a second fluid or particulate are disclosed. The devices include an acoustic chamber, a fluid outlet at a top end of the acoustic chamber, a concentrate outlet at a bottom end of the acoustic chamber, and an inlet on a first side end of the acoustic chamber. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the acoustic chamber that traps and separates particulates (e.g. cells) from a host fluid. The host fluid is collected via the fluid outlet, and the particulates are collected via the concentrate outlet. The device is a large-scale device that is able to process liters/hour, and has a large interior volume.

Abstract: A baggage restraint system for the containment of luggage and other personal or group articles, such as camping gear or sport equipment, to the confines of two or more successive seats in a commercial passenger vehicle, such as a school bus. The system comprises an enclosure, and the means by which it may be releasably secured to the vehicle cabin floor. The system's enclosure is constructed of tear-resistant fabric panels, as an essentially rectangular or forward-leaning, four-sided cuboid. The enclosure is shaped and dimensioned to envelope a predetermined number of seats in a target vehicle. The system's means of securement include: a plurality of depending loops, affixed along one or more of the enclosure's bottom and windows-side edges; a plurality of floor and sidewall anchor tabs; and a plurality of carabiners and adjustable straps, linking each anchor tab to one of the depending loops.

Abstract: An acoustophoresis device made up of modular components is disclosed. Several modules are disclosed herein, including ultrasonic transducer modules, input/output modules, collection well modules, and various connector modules. These permit different systems to be constructed that have appropriate fluid dynamics for separation of particles, such as biological cells, from a fluid.