Patents by Inventor Benjamin Hindson

Benjamin Hindson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Instrument systems for integrated sample processing
    Patent number: 10245587
    Abstract: An integrated system for processing and preparing samples for analysis may include a microfluidic device including a plurality of parallel channel networks for partitioning the samples including various fluids, and connected to a plurality of inlet and outlet reservoirs, at least a portion of the fluids comprising reagents, a holder including a closeable lid hingedly coupled thereto, in which in a closed configuration, the lid secures the microfluidic device in the holder, and in an open configuration, the lid is a stand orienting the microfluidic device at a desired angle to facilitate recovery of partitions or droplets from the partitioned samples generated within the microfluidic device, and an instrument configured to receive the holder and apply a pressure differential between the plurality of inlet and outlet reservoirs to drive fluid movement within the channel networks.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: April 2, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Donald A. Masquelier, Benjamin Hindson, Kevin Ness, Rajiv Bharadwaj
  • Methods and Systems for Processing Polynucleotides
    Publication number: 20190085391
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.
    Type: Application
    Filed: October 19, 2018
    Publication date: March 21, 2019
    Inventors: Benjamin Hindson, Mima Jarosz, Paul Hardenbol, Michael Schnall-Levin, Kevin Ness, Serge Saxonov
  • Methods and systems for processing polynucleotides
    Patent number: 10227648
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: March 12, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol
  • Compositions and methods for sample processing
    Patent number: 10221442
    Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
    Type: Grant
    Filed: June 26, 2014
    Date of Patent: March 5, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
  • Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
    Patent number: 10221436
    Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.
    Type: Grant
    Filed: January 7, 2016
    Date of Patent: March 5, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Paul Hardenbol, Pranav Patel, Benjamin Hindson, Paul William Wyatt, Keith Bjornson, Indira Wu, Kamila Belhocine
  • Methods and systems for processing polynucleotides
    Patent number: 10208343
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: December 5, 2017
    Date of Patent: February 19, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Xinying Zheng, Phillip Belgrader
  • Methods and Systems for Processing Polynucleotides
    Publication number: 20190032129
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: September 27, 2018
    Publication date: January 31, 2019
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Serge Saxonov, Rajiv Bharadwaj
  • MAGNETIC LYSIS METHOD AND DEVICE
    Publication number: 20190024039
    Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
    Type: Application
    Filed: September 24, 2018
    Publication date: January 24, 2019
    Inventors: Phillip BELGRADER, Benjamin HINDSON
  • Methods and Systems for Processing Polynucleotides
    Publication number: 20190024166
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: September 21, 2018
    Publication date: January 24, 2019
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20190017115
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: May 15, 2018
    Publication date: January 17, 2019
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mima Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • METHODS AND SYSTEMS FOR SAMPLE PROCESSING POLYNUCLEOTIDES
    Publication number: 20180371540
    Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
    Type: Application
    Filed: August 1, 2018
    Publication date: December 27, 2018
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Donald Masquelier, Serge Saxonov, Landon Merrill, Andrew Price, Paul Hardenbol, Yuan Li
  • COMPOSITIONS AND METHODS FOR SAMPLE PROCESSING
    Publication number: 20180363029
    Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
    Type: Application
    Filed: August 6, 2018
    Publication date: December 20, 2018
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Donald Masquelier, Serger Saxonov, Landon Merrill, Andrew Price, Paul Hardenbol, Yuan Li
  • Partitioning and processing of analytes and other species
    Patent number: 10150963
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: March 27, 2017
    Date of Patent: December 11, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Christopher Hindson, Donald Masquelier, Mirna Jarosz, Michael Schnall-Levin
  • Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
    Patent number: 10150117
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: December 11, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Partitioning and processing of analytes and other species
    Patent number: 10150964
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: December 11, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Christopher Hindson, Donald Masquelier, Mirna Jarosz, Michael Schnall-Levin
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20180346979
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: August 1, 2018
    Publication date: December 6, 2018
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
    Patent number: 10137449
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: November 27, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Magnetic lysis method and device
    Patent number: 10138458
    Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: November 27, 2018
    Assignee: Akonni Biosystems, Inc.
    Inventors: Phillip Belgrader, Benjamin Hindson
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20180327839
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: July 25, 2018
    Publication date: November 15, 2018
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20180282804
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: June 5, 2018
    Publication date: October 4, 2018
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Michael Schnall-Levin, Mirna Jarosz