Abstract: The present disclosure provides methods to transfer a pressurized slurry of plant-based biomass into a reactor vessel. The methods allow for the transfer of practical-sized pieces of biomass in a slurry pressurized to above about 300 psi.

Abstract: The present disclosure provides methods to transfer a pressurized slurry of plant-based biomass into a reactor vessel. The methods allow for the transfer of practical-sized pieces of biomass in a slurry pressurized to above about 300 psi.

Abstract: The present disclosure provides a solid lignocellulosic hydrolysate and methods to prepare the solid lignocellulosic hydrolysate from a woody biomass or an herbaceous biomass. The solid lignocellulosic hydrolysate may be used in the production of biofuels, bioproducts, and food products. The solid lignocellulosic hydrolysate allows for ease of storage, ease of transportation and handling of the solid lignocellulosic hydrolysate, and ease of use in biological or fermentation processes or chemical processes for the production of biofuel, bioproducts, chemicals and food products due to the bulk handling characteristics (e.g., solubility and rate of dissolution) of the solid lignocellulosic hydrolysate.

Abstract: The present invention provides spray methods of delivering saccharification enzymes, fermentation organisms, and other hydrolysis or fermentation ingredients onto lignocellulosic biomass. The methods reduce the need for mechanical mixing when the biomass solids are undergoing enzymatic hydrolysis, and reduce dilution to allow higher product titers in the hydrolysis and/or fermentation steps.

Abstract: The present invention provides methods of producing pretreated lignocellulosic biomass combined with enzymes for the storage and transporation of the pretreated lignocellulosic biomass that may be used in biofuel and bioproduct production. The methods allows the coexistence of the pretreated lignocellulosic biomass and the enzymes during storage and transporation, the immediate hydrolysis of the pretreated lignocellulosic biomass to produce sugars, without further addition of enzymes, in a biofuel or bioproduct production site, the enhancement of the final hydrolytic activity of the pretreated lignocellulosic biomass, and/or the reduction in sensitivity of the inhibitors in the pretreated lignocellulosic biomass.

Abstract: The present invention provides spray methods of delivering saccharification enzymes, fermentation organisms, and other hydrolysis or fermentation ingredients onto lignocellulosic biomass. The methods reduce the need for mechanical mixing when the biomass solids are undergoing enzymatic hydrolysis, and reduce dilution to allow higher product titers in the hydrolysis and/or fermentation steps.

Abstract: Provided are methods for the pretreatment of ligno-cellulosic biomass such as softwoods with bisulfite such as ammonium bisulfite without the need for exogenous acid. In one variation, a method of pretreating ligno-cellulosic biomass is provided including the following steps: a) providing ligno-cellulosic biomass; b) contacting the ligno-cellulosic biomass with a solution comprising bisulfite at an amount between 1 and 10% of a dry weight of the ligno-cellulosic biomass to form a slurry; c) heating the slurry to a first temperature of 150-210° C. for a first period of time to form a first mixture; d) cooling the first mixture to a second temperature of 100-200° C. to form a second mixture; and e) maintaining the second mixture at the second temperature for a second period of time to form pretreated ligno-cellulosic biomass; wherein the first temperature is higher than the second temperature.