Patents by Inventor Bernd Buchberger
Bernd Buchberger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11098298Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: May 1, 2019Date of Patent: August 24, 2021Assignee: BIOMÉRIEUX DEUTSCHLAND GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Patent number: 11092592Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: GrantFiled: January 30, 2020Date of Patent: August 17, 2021Assignee: BIOMÉRIEUX DEUTSCHLAND GMBHInventor: Bernd Buchberger
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Publication number: 20200166500Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: January 30, 2020Publication date: May 28, 2020Applicant: HYGLOS INVEST GMBHInventor: Bernd BUCHBERGER
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Patent number: 10655113Abstract: The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonisation and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.Type: GrantFiled: April 22, 2019Date of Patent: May 19, 2020Assignee: HYPHARM GMBHInventors: Anna Scherzinger, Sonja Molinaro, Bernd Buchberger
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Patent number: 10585086Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: GrantFiled: June 12, 2015Date of Patent: March 10, 2020Assignee: HYGLOS INVEST GMBHInventor: Bernd Buchberger
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Publication number: 20190256836Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: ApplicationFiled: May 1, 2019Publication date: August 22, 2019Applicant: HYGLOS INVEST GMBHInventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
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Publication number: 20190249156Abstract: The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonisation and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.Type: ApplicationFiled: April 22, 2019Publication date: August 15, 2019Applicant: HYPHARM GMBHInventors: Anna SCHERZINGER, Sonja MOLINARO, Bernd BUCHBERGER
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Patent number: 10323238Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: June 28, 2017Date of Patent: June 18, 2019Assignee: HYGLOS INVEST GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Patent number: 10301607Abstract: The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonization and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.Type: GrantFiled: January 20, 2016Date of Patent: May 28, 2019Assignee: HYPHARM GMBHInventors: Anna Scherzinger, Sonja Molinaro, Bernd Buchberger
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Publication number: 20170306315Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: ApplicationFiled: June 28, 2017Publication date: October 26, 2017Applicant: HYGLOS INVEST GMBHInventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
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Publication number: 20170227529Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: ApplicationFiled: June 12, 2015Publication date: August 10, 2017Applicant: HYGLOS INVEST GMBHInventor: Bernd BUCHBERGER
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Patent number: 9725706Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harboring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: December 4, 2013Date of Patent: August 8, 2017Assignee: HYGLOS INVEST GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Patent number: 9453842Abstract: The present invention deals with reagents and compositions capable of effectively inhibiting peroxidase activity. According to the invention, peroxidase enzymatic activity is blocked with an acidic aqueous solution of a protein denaturing agent. Preferred protein denaturing agents are detergents and chaotropic substances.Type: GrantFiled: April 9, 2014Date of Patent: September 27, 2016Assignee: Roche Molecular Systems, Inc.Inventors: Manfred Watzele, Bernd Buchberger, Claudia Kirr
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Publication number: 20160137993Abstract: The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonisation and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.Type: ApplicationFiled: January 20, 2016Publication date: May 19, 2016Applicant: HYGLOS INVEST GMBHInventors: Anna Scherzinger, Sonja Molinaro, Bernd Buchberger
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Patent number: 9249447Abstract: The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonization and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.Type: GrantFiled: June 17, 2011Date of Patent: February 2, 2016Assignee: HYGLOS INVEST GMBHInventors: Anna Scherzinger, Sonja Molinaro, Bernd Buchberger
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Publication number: 20150299684Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: ApplicationFiled: December 4, 2013Publication date: October 22, 2015Applicant: MICROCOAT BIOTECHNOLOGIE GMBHInventors: Bernd BUCHBERGER, Holger GRALLERT, Sonja MOLINARO
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Patent number: 8951780Abstract: The invention concerns a method for isolating and purifying nucleic acids from large sample volumes and a device suitable for this. The device comprises a funnel, a separating column, and a tab which are detachable from each other. The method according to the invention in which the device according to the invention is used ensures a higher yield of nucleic acids.Type: GrantFiled: October 30, 2008Date of Patent: February 10, 2015Assignee: Roche Molecular Systems, Inc.Inventors: Maximilian Ruhstorfer, Bernd Buchberger, Horst Donner, Alois Rainer
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Publication number: 20140220593Abstract: The present invention deals with reagents and compositions capable of effectively inhibiting peroxidase activity. According to the invention, peroxidase enzymatic activity is blocked with an acidic aqueous solution of a protein denaturing agent. Preferred protein denaturing agents are detergents and chaotropic substances.Type: ApplicationFiled: April 9, 2014Publication date: August 7, 2014Inventors: Manfred WATZELE, Bernd BUCHBERGER, Claudia KIRR
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Patent number: 8735083Abstract: The present invention deals with reagents and compositions capable of effectively inhibiting peroxidase activity. According to the invention, peroxidase enzymatic activity is blocked with an acidic aqueous solution of a protein denaturing agent. preferred protein denaturing agents are detergents and chaotropic substances.Type: GrantFiled: June 6, 2007Date of Patent: May 27, 2014Assignee: Roche Diagnostics Operations, Inc.Inventors: Manfred Watzele, Bernd Buchberger, Claudia Kirr
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Publication number: 20130288926Abstract: The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonisation and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.Type: ApplicationFiled: June 17, 2011Publication date: October 31, 2013Applicant: HYGLOS INVEST GMBHInventors: Anna Scherzinger, Sonja Molinaro, Bernd Buchberger