Abstract: The present invention relates to a process for the biocatalytic, enantioselective production of a ?-amino acid pre-cursor from an optionally substituted dihydrouracil using a hydantoinase and/or a dihydropyrimidinase, a process for producing a ?-amino acid from said precursor, a hydantoinase and its use in said process for the biocatalytic production of a ?-amino acid pre-cursor or a ?-amino acid, and a method for obtaining said hydantoinase.

Abstract: The present invention relates to a process for preparing (meth)acrylic esters of alcohols having C—C triple bonds, to processes for their preparation, and to their use.

Abstract: Provided is a process for esterifying or transesterifying side chains in polymers, which involves esterifying or transesterifying the carboxyl group having the R4 radical of a copolymer obtained by polymerizing a (meth)acrylate of formula (I), and a monomer of formula (II): wherein R1 and R3 are independently H or CH3, R2 and R4 are independently H or an alkyl radical which is optionally substituted by functional groups such as acryloyl, ether, amino, epoxy, halogen or sulfonic acid groups, and n is from 1 to 40, with an alcohol in the presence of an enzyme which catalyzes the esterifying or transesterifying. Further provided is the esterified or transesterified polymer product and its use.

Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.

Abstract: The present invention relates to a process for the production of a polyamine involving the use of enzymes; in particular to a process performed in aqueous environment; to the polyamines produced by said method; as well as the use of said polyamines for manufacturing paper, for immobilizing enzymes, or for preparing pharmaceutical or cosmetical compositions. The invention also relates to a novel method for in situ regeneration of cofactors NAD(P)+.

Abstract: The invention relates to a method for the enzymatic reduction of alkyne derivatives of the formula (1), wherein R1 represents H, C1-C6-alkyl, C2-C6-alkenyl, or an optionally substituted carbocyclic or heterocyclic, aromatic or non-aromatic group, R2 represents H, C1-C6-alkyl, C2-C6-alkenyl, by reaction in the presence of special reductases.

Abstract: The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacyl-glycerol lipase mutants, as for example derived from Candida antarctica lipase B (CALB); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences.

Abstract: A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.

Abstract: A process for the production of an optically enriched tertiary alcohol of the formula (2a) or (2b), by reacting an epoxide of the formula (1) with a nucleophilic agent Nu in the presence of halohydrin dehalogenase.

Abstract: The invention relates to a process for the biocatalytic production of ambroxan by means of a polypeptide with the activity of a homofarnesol-ambroxan cyclase, which are a novel class of enzymes.

Abstract: The present invention relates to a method for the enzymatic hydroamination of C—C double bonds catalyzed by enzymes structurally and/or functionally related to phenylalanine ammonia lyase (PAL) isolated from microorganisms of Petroselinum crispum, Rhodoturula glutinis and/or functional active derivatives thereof.

Abstract: A process for the racemization of an optically active alpha-hydroxyketone by incubating said alpha-hydroxyketone in the presence of an acetoin racemase of Lactobacillus.

Abstract: The invention relates to a process for the production of optically active Citronellal by enzymatic reduction of Citral with a reductase from Zymomonas mobilis.

Abstract: The present invention relates to enzymic processes for preparing S-butan-2-ol; and to enzymes for carrying out said processes; to nucleic acid sequences coding for said enzymes, to expression cassettes, vectors and recombinant hosts containing said nucleic acid sequences.

Abstract: The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

Abstract: A process for the production of an optically enriched oxazolidinone of the formula (2a) or (2b), by reacting an epoxide of the formula (1) with cyanate in the presence of halo-hydrin dehalogenase.

Abstract: The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

Abstract: The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.

Abstract: The present invention relates to a multi-stage process for producing substituted, optically active alcohols, comprising an enzyme-catalyzed synthesis step, in particular a synthesis step which is catalyzed by an alcohol dehydrogenase. The inventive method is particularly suitable for producing phenylephrine, i.e. 3-[(1R)-1-hydroxy-2-methylamino-ethyl]-phenol.

Abstract: The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.