Abstract: A multilayered liposome disclosed herein includes a liposome core defined by a lipid layer, and five or more coating layers surrounding the lipid layer, wherein the five or more coating layers include more than one positively charged polymeric layer and more than one negatively charged drug layer, wherein the more than one positively polymeric layer and the more than one negatively charged drug layer are deposited in an alternating manner, wherein one of the more than one positively charged polymeric layer is formed as an outmost coating layer, and wherein each of the more than one negatively charged drug layer includes insulin, an insulin-like factor, a growth factor, or a hormonal peptide. In one embodiment, an anion liposome core (HSPC/DPPG) is coated via a layer-by-layer approach with multilayers of oppositely charged insulin and chitosan layers.

Abstract: This invention relates to a method for separating blood cells comprising the steps of: (a) lysing red blood cells of a blood sample and diluting said sample; (b) providing a microfluidic device comprising a spiral-shaped flow channel having at least a first end and a second end, wherein said flow channel has two inlet ports at or near said first end and at least two outlet ports at or near said second end, wherein one of the two inlet ports is located at the inner wall of the spiral-shaped flow channel and the other inlet port is located at the outer wall of the spiral-shaped flow channel and at least one of the outlet ports is connected to a container allowing the storage of blood cells; (c) introducing the sample of step (a) into the inlet port located at the outer wall of the spiral-shaped flow channel and introducing a sheath fluid into the inlet port located at the inner wall of the spiral-shaped flow channel; (d) driving said sample and the sheath fluid through the spiral-shaped flow channel; and (e) re

Abstract: This invention relates to a method for separating blood cells comprising the steps of: (a) lysing red blood cells of a blood sample and diluting said sample; (b) providing a microfluidic device comprising a spiral-shaped flow channel having at least a first end and a second end, wherein said flow channel has two inlet ports at or near said first end and at least two outlet ports at or near said second end, wherein one of the two inlet ports is located at the inner wall of the spiral-shaped flow channel and the other inlet port is located at the outer wall of the spiral-shaped flow channel and at least one of the outlet ports is connected to a container allowing the storage of blood cells; (c) introducing the sample of step (a) into the inlet port located at the outer wall of the spiral-shaped flow channel and introducing a sheath fluid into the inlet port located at the inner wall of the spiral-shaped flow channel; (d) driving said sample and the sheath fluid through the spiral-shaped flow channel; and (e) re