Abstract: A method of electrically coupling an electrode pair in a plurality of electrode pairs in a biosensor with a macromolecule. The electrode pair comprises a first electrode and a second electrode. A first portion of the macromolecule is derivatized with a first reactive group and a second portion of the macromolecule is derivatized with a second reactive group. The first reactive group is masked with a first electrolabile masking group and the second reactive group is masked with a second electrolabile masking group. A first voltage is applied at the first electrode in the electrode pair under conditions that are sufficient to unmask the first reactive group. The unmasked first reactive group binds to the first electrode thereby linking the macromolecule to the first electrode. A second voltage is applied at the second electrode in the electrode pair under conditions that are sufficient to unmask the second reactive group.