Patents by Inventor Bhupendra Vallabh Kara
Bhupendra Vallabh Kara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220213500Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: ApplicationFiled: March 15, 2022Publication date: July 7, 2022Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Patent number: 11306323Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: GrantFiled: December 12, 2017Date of Patent: April 19, 2022Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Publication number: 20210348205Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: ApplicationFiled: July 16, 2021Publication date: November 11, 2021Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Patent number: 11098335Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: GrantFiled: May 8, 2017Date of Patent: August 24, 2021Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Patent number: 10465220Abstract: A process for the production of a target recombinant polypeptide is provided. The process comprises expressing an expression cassette encoding the target polypeptide and co-expressing an expression cassette encoding a mitochondrial foldase. Preferred mitochondrial foldases include Mia 40 and Erv1, and homologs thereof.Type: GrantFiled: November 2, 2011Date of Patent: November 5, 2019Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
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Publication number: 20180127777Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: ApplicationFiled: December 12, 2017Publication date: May 10, 2018Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Publication number: 20180057823Abstract: An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.Type: ApplicationFiled: November 15, 2017Publication date: March 1, 2018Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
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Patent number: 9873891Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: GrantFiled: April 29, 2014Date of Patent: January 23, 2018Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Patent number: 9845475Abstract: An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.Type: GrantFiled: October 12, 2015Date of Patent: December 19, 2017Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
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Publication number: 20170240943Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: ApplicationFiled: May 8, 2017Publication date: August 24, 2017Applicant: Fujifilm Diosynth BiotechnologiesInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Patent number: 9677103Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: GrantFiled: August 14, 2013Date of Patent: June 13, 2017Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Publication number: 20160076051Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: ApplicationFiled: April 29, 2014Publication date: March 17, 2016Applicant: FUJIFILM Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Publication number: 20160024509Abstract: An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.Type: ApplicationFiled: October 12, 2015Publication date: January 28, 2016Applicant: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LIMITEDInventors: Bhupendra Vallabh KARA, Christopher David John LENNON
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Patent number: 9157085Abstract: An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.Type: GrantFiled: June 1, 2009Date of Patent: October 13, 2015Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
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Patent number: 9051576Abstract: A recombinant yeast of the genus Komagataella which has been transformed with a galactose promoter is provided. The yeast is preferably Komagataella phaffii, and the promoter is preferably a S. cerevisiae GAL1 or GAL10 promoter. Vectors and methods for producing recombinant polypeptides are also provided.Type: GrantFiled: July 26, 2012Date of Patent: June 9, 2015Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventor: Bhupendra Vallabh Kara
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Publication number: 20140370543Abstract: A process for the production of a target recombinant polypeptide is provided. The process comprises expressing an expression cassette encoding the target polypeptide and co-expressing an expression cassette encoding a mitochondrial foldase. Preferred mitochondrial foldases include Mia 40 and Erv1, and homologs thereof.Type: ApplicationFiled: November 2, 2011Publication date: December 18, 2014Applicant: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LIMITEDInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
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Publication number: 20140162315Abstract: A recombinant yeast of the genus Komagataella which has been transformed with a galactose promoter is provided. The yeast is preferably Komagataella phaffii, and the promoter is preferably a S. cerevisiae GAL1 or GAL10 promoter. Vectors and methods for producing recombinant polypeptides are also provided.Type: ApplicationFiled: July 26, 2012Publication date: June 12, 2014Applicant: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LIMITEDInventor: Bhupendra Vallabh Kara
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Publication number: 20140065670Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: ApplicationFiled: August 14, 2013Publication date: March 6, 2014Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Patent number: 8530188Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: GrantFiled: February 1, 2007Date of Patent: September 10, 2013Assignee: Fujifilm Diosynth Biotechnologies (UK) LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Patent number: 8394937Abstract: A protein expression system is provided. The system comprises: a) a T7 RNA polymerase-dependent promoter operably linked to an expression cassette for a protein of interest; and b) an expression cassette for T7 RNA polymerase operably linked to a ?pL promoter and at least two perfect palindrome operator sequences.Type: GrantFiled: May 8, 2008Date of Patent: March 12, 2013Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Ian John Hodgson