Abstract: A lattice light sheet microscope includes a single spatial light modulator (SLM), the optical modulation plane of which conjugates to an image plane of an excitation objective and which is configured to: generate an optical lattice by loading a phase map obtained from a central cross section of a corresponding optical lattice of a desired lattice light sheet, and tile the optical lattice by loading a phase map obtained from an off-center cross section of the corresponding optical lattice. The lattice light sheet microscope also includes a transparent annular diaphragm arranged at a plane conjugating to the entrance pupil of the excitation objective lens. The lattice light sheet microscope further includes a first galvanometer configured to: scan each optical lattice in the extending direction thereof so as to form a tiled lattice light sheet.

Abstract: Matrix-assisted methods and compositions, including those based on solutions containing low melting agarose, to prepare intact organs and other samples for super resolution imaging by microscopy, and more particularly, lightsheet microscopy.

Abstract: The present disclosure relates to a lattice light sheet microscope and a method for tiling a lattice light sheet in the lattice light sheet microscope. The lattice light sheet microscope comprises: a single spatial light modulator (SLM), the optical modulation plane of which conjugates to an image plane of an excitation objective lens and which is configured to: generate an optical lattice by loading a phase map obtained from a central cross section of a corresponding optical lattice of a desired lattice light sheet, and tile the optical lattice by loading a phase map obtained from an off-center cross section of the corresponding optical lattice; a transparent annular diaphragm, arranged at a plane conjugating to the entrance pupil of the excitation objective lens; and a first galvanometer, configured to: scan each optical lattice in the extending direction thereof so as to form a tiled lattice light sheet.