Abstract: Methods of generating mature ssDNA LASSO probes using DNA recombinase mediated assembly are provided. Also provided are mature ssDNA LASSO probes made by the methods, methods of their use, and kits including such.

Abstract: Methods and compositions for use in engineering cells to secrete therapeutic biomolecules into the blood stream in vivo in response to an individual's personal biological needs.

Abstract: Systems and methods for the dynamic co-culturing of two cell populations are provided. The system includes a barrier configured to physically separate a stimulator cell population from a responder cell population disposed within a container. The barrier is permeable to the secreted factors of at least one of the cell populations. The responder cell population can thereby be altered by exposure to the secreted factors to produce a population of reprogrammed cells that includes biomolecules (e.g., nucleic acids) originating from the stimulator cell population and/or that exhibits one or more additional or modified functional activities than a parental population of the reprogrammed cells.

Abstract: Long adapter single strand oligonucleotide (LASSO) probes that can be used to capture and clone thousands of kilobase-sized DNA fragments in a single reaction, as well as methods of generating the same.

Abstract: Systems and methods for growing cells are provided. A capsule for growing or storing cells includes a shell defining an interior compartment and a substrate for cell attachment located within the compartment. The substrate comprises a polymer and one or more adhesion molecules. The substrate for cell attachment can be an inner surface of the shell and/or a hydrogel disposed within the interior compartment.

Abstract: A method for suppressing an immune response is provided. The method involves administration of isolated lymphoid tissue-derived suppressive stromal cells (LSSC) to a subject in need of such treatment in an amount effective to suppress the immune response in the subject. The invention also involves a method to isolate LSSC by digesting lymphoid tissue fragments using a combination of an enzyme mix and agitation and then collecting the LSSC. Pharmaceutical preparations comprising LSSC are also provided.

Abstract: A method for suppressing an immune response is provided. The method involves administration of isolated lymphoid tissue-derived suppressive stromal cells (LSSC) to a subject in need of such treatment in an amount effective to suppress the immune response in the subject. The invention also involves a method to isolate LSSC by digesting lymphoid tissue fragments using a combination of an enzyme mix and agitation and then collecting the LSSC. Pharmaceutical preparations comprising LSSC are also provided.

Abstract: Described are compositions and methods for treating liver disease, e.g., acute liver disease, using bone marrow-derived stem cells and bone marrow-derived stem cell conditioned media.

Abstract: Long adapter single strand oligonucleotide (LASSO) probes that can be used to capture and clone thousands of kilobase-sized DNA fragments in a single reaction, as well as methods of generating the same.

Abstract: A system and accompanying methods provide for the creation and navigation of interactive, three-dimensional media presentations. A three-dimensional presentation is received, and includes a three-dimensional canvas having integrated therein a plurality of two-dimensional content elements from a first content source and a plurality of three-dimensional content elements from a second content source. The two-dimensional content elements and the three-dimensional content elements relate to a common topic. The three-dimensional presentation is displayed to a user on a user device by moving a virtual camera about the three-dimensional canvas over a plurality of defined motion paths. While displaying the three-dimensional presentation to the user, user navigation instructions with respect to a first one of the integrated content elements are received.

Abstract: Described are compositions and methods for treating liver disease, e.g., acute liver disease, using bone marrow-derived stem cells and bone marrow-derived stem cell conditioned media.

Abstract: A method for suppressing an immune response is provided. The method involves administration of isolated lymphoid tissue-derived suppressive stromal cells (LSSC) to a subject in need of such treatment in an amount effective to suppress the immune response in the subject. The invention also involves a method to isolate LSSC by digesting lymphoid tissue fragments using a combination of an enzyme mix and agitation and then collecting the LSSC. Pharmaceutical preparations comprising LSSC are also provided.

Abstract: The present invention provides particular anti-inflammatory factors, compositions containing them, methods of making, identifying, and/or characterizing them, and methods of using them. In some embodiments, provided factors are expressed by human bone marrow stromal cells (MSC). In some embodiments, provided factors are characterized by an ability, when contacted with mammalian leukocytes in culture, to alter production of at least one inflammatory or anti-inflammatory agent by the mammalian leukocytes. In some embodiments, provided factors include GALNT1 polypeptides, LGALS3BP polypeptides, MFAP5 polypeptides, PENK polypeptides and/or HAPLN1 polypeptides. In some embodiments, provided factors are useful in the inhibition of inflammatory agents, in the promotion of anti-inflammatory agents, and/or for the treatment of subjects suffering from or susceptible to a disease, disorder or condition characterized by inflammation.

Abstract: Described are compositions and methods for treating liver disease, e.g., acute liver disease, using bone marrow-derived stem cells and bone marrow-derived stem cell conditioned media.

Abstract: The invention provides compositions and methods for modulating disease using hematopoietic stromal progenitor cells derived from hematopoietic tissues. The invention further provides methods for isolating, storing and culturing hematopoietic stromal progenitor cells.

Abstract: Described are compositions and methods for treating liver disease, e.g., acute liver disease, using bone marrow-derived stem cells and bone marrow-derived stem cell conditioned media.

Abstract: The invention provides compositions and methods for modulating immune responses using mesenchymal stem cells. The invention further provides methods for inducing tolerance to self antigens using mesenchymal stem cells.

Abstract: Described are compositions and methods for treating liver disease, e.g., acute liver disease, using bone marrow-derived stem cells and bone marrow-derived stem cell conditioned media.