Abstract: An analysis cartridge and an analysis method, the analysis cartridge includes a first cover, a second cover, containers, a fluid tunnels and a rotary valve. The second cover has two opposite surfaces, a plurality of first through holes and a second through hole individually penetrate through the two opposite surfaces, and the first cover is attached to the second cover. The plurality of containers are disposed between the first cover and the second cover, with each of the containers being aligned to and filled in the first through holes. The plurality of the fluid tunnels is disposed on the first cover, and each of which is individually connected with a first pipette. The rotary valve is rotably disposed between the first cover and the second cover to correspond to the second through hole, and a flow channel disposed on the rotary valve is connected with the containers individually.