Abstract: Provided herein are high potency sweeteners containing at least 95% rebaudioside M generated from Saccharomyces cerevisiae strains engineered to produce high purity rebaudioside M when fermented with sugar cane syrup. In addition, provided are methods of purifying the high potency sweeteners from cleared fermentation broths. Also provided are sugar substitutes containing the high potency sweeteners and methods of making same.

Abstract: Methods of producing renewable materials may include consuming a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth; water may then be separated from the feedstock or broth using one or more phase separations, or the renewable material may be concentrated from the feedstock or broth using one or more phase separations. Methods of producing biofuel components may include consuming a lignocellulosic or sugar fermentation feedstock with a fermentation organism to produce either ethanol or butanol in fermentation broth; cooling the feedstock or broth to solidify at least some water therein; and separating the solidified water from the feedstock or broth using a solid-liquid phase separation.