Patents by Inventor Birgit Brucher
Birgit Brucher has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230193220Abstract: The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.Type: ApplicationFiled: July 12, 2022Publication date: June 22, 2023Inventors: Christopher David BAYER, Andreas VOGEL, Marc STRUHALLA, Birgit BRUCHER
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Patent number: 11674129Abstract: The present invention relates to polypeptide, preferably to a glucose isomerase, comprising an amino acid sequence, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, is at least 95% identical to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are each and independently selected from the group consisting of SEQ ID NO: 1 amino acid positions 89, 90, 95, 0, 33, 34, 35, and 59. The present invention further relates to methods preparing glucose including the use of the polypeptides of the invention for preparing glucose.Type: GrantFiled: June 9, 2021Date of Patent: June 13, 2023Assignees: NEW MATTERHORN, LLC, C-LEcta GmbHInventors: Birgit Brucher, Andreas Vogel, Hanna Maria Dudek, Rico Czaja
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Patent number: 11421207Abstract: The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.Type: GrantFiled: November 28, 2018Date of Patent: August 23, 2022Assignees: C-Lecta GmbH, New Matterhorn, LLCInventors: Christopher David Bayer, Andreas Vogel, Marc Struhalla, Birgit Brucher
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Patent number: 11286474Abstract: The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.Type: GrantFiled: November 13, 2019Date of Patent: March 29, 2022Assignee: C-LECTA GMBHInventors: Stefan Schoenert, Mathias Salomo, Thomas Schultchen, Andreas Vogel, Sabrina Koepke, Sebastian Bartsch, Birgit Brucher, Claudia Feller
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Publication number: 20210380954Abstract: The present invention is related to a trehalose phosphorylase comprising an amino acid sequence, wherein the amino acid sequence of the trehalose phosphorylase is at least 80% identical to and/or at least 80% homologous to an amino acid sequence of SEQ ID NO: 1, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are selected from the group consisting of amino acid positions of SEQ ID NO: 1712, 383, 10, 114, 118, 192, 197, 220, 225, 304, 306, 318, 323, 339, 349, 357, 459, 476, 481, 484, 487, 488, 506, 511, 526, 530, 532, 533, 537, 550, 556, 564, 590, 649, 667, 703 and 705.Type: ApplicationFiled: June 10, 2021Publication date: December 9, 2021Inventors: Birgit Brucher, Andreas Vogel
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Publication number: 20210317432Abstract: The present invention relates to polypeptide, preferably to a glucose isomerase, comprising an amino acid sequence, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, is at least 95% identical to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are each and independently selected from the group consisting of SEQ ID NO: 1 amino acid positions 89, 90, 95, 0, 33, 34, 35, and 59. The present invention further relates to methods preparing glucose including the use of the polypeptides of the invention for preparing glucose.Type: ApplicationFiled: June 9, 2021Publication date: October 14, 2021Inventors: Birgit Brucher, Andreas Vogel, Hanna Maria Dudek, Rico Czaja
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Patent number: 11142749Abstract: The present invention is related to a trehalose phosphorylase comprising an amino acid sequence, wherein the amino acid sequence of the trehalose phosphorylase is at least 80% identical to and/or at least 80% homologous to an amino acid sequence of SEQ ID NO: 1, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are selected from the group consisting of amino acid positions of SEQ ID NO: 1 712, 383, 10, 114, 118, 192, 197, 220, 225, 304, 306, 318, 323, 339, 349, 357, 459, 476, 481, 484, 487, 488, 506, 511, 526, 530, 532, 533, 537, 550, 556, 564, 590, 649, 667, 703 and 705.Type: GrantFiled: November 28, 2017Date of Patent: October 12, 2021Assignees: NEW MATTERHORN, LLC, c-LEcta GmbHInventors: Birgit Brucher, Andreas Vogel
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Patent number: 11066659Abstract: The present invention relates to polypeptide, preferably to a glucose isomerase, comprising an amino acid sequence, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, is at least 95% identical to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are each and independently selected from the group consisting of SEQ ID NO: 1 amino acid positions 89, 90, 95, 0, 33, 34, 35, and 59. The present invention further relates to methods preparing glucose including the use of the polypeptides of the invention for preparing glucose.Type: GrantFiled: May 4, 2018Date of Patent: July 20, 2021Assignees: NEW MATTERHORN, LLC, C-LEcta GmbHInventors: Birgit Brucher, Andreas Vogel, Hanna Maria Dudek, Rico Czaja
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Patent number: 11021691Abstract: The present invention relates to a sucrose phosphorylase variant comprising or consisting of an amino acid sequence with at least 75% homologous and/or identical to an amino acid sequence of wild type sucrose phosphorylase, preferably wherein the sucrose phosphorylase is characterized by different structural and functional features. The invention also relates to method for preparing aG1P and a co-product, wherein the method comprises reacting a sucrose phosphorylase with a glucoside substrate, wherein the sucrose phosphorylase is a sucrose phosphorylase as defined in the context of the present invention.Type: GrantFiled: November 28, 2018Date of Patent: June 1, 2021Assignees: New Matterhorn, LLC, c-LEcta GmBHInventors: Claudia Feller, Birgit Brucher, Andreas Vogel
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Publication number: 20210087598Abstract: The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.Type: ApplicationFiled: November 28, 2018Publication date: March 25, 2021Inventors: Christopher David Bayer, Andreas Vogel, Marc Struhalla, Birgit Brucher
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Publication number: 20200377867Abstract: The present invention relates to a sucrose phosphorylase variant comprising or consisting of an amino acid sequence with at least 75% homologous and/or identical to an amino acid sequence of wild type sucrose phosphorylase, preferably wherein the sucrose phosphorylase is characterized by different structural and functional features. The invention also relates to method for preparing aG1P and a co-product, wherein the method comprises reacting a sucrose phosphorylase with a glucoside substrate, wherein the sucrose phosphorylase is a sucrose phosphorylase as defined in the context of the present invention.Type: ApplicationFiled: November 28, 2018Publication date: December 3, 2020Inventors: Claudia Feller, Birgit Brucher, Andreas Vogel
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Publication number: 20200347424Abstract: The invention relates to a saccharose phosphorylase that catalyzes the synthesis of glucose-1-phosphate and fructose from saccharose and phosphate, among other things. The saccharose phosphorylase according to the invention can be considered to be a mutation of the saccharose phosphorylase from Bifidobacterium adolescentis. In comparison to wild-type saccharose phosphorylase, the saccharose phosphorylase according to the invention is distinguished by improved activity, process stability, temperature stability, and lower product inhibition and is therefore particularly suitable for use in industrial processes.Type: ApplicationFiled: March 7, 2017Publication date: November 5, 2020Inventors: Timo Johannes Koch, Thomas Hässler, Birgit Brucher, Andreas Vogel
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Publication number: 20200224187Abstract: The present invention relates to polypeptide, preferably to a glucose isomerase, comprising an amino acid sequence, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, is at least 95% identical to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are each and independently selected from the group consisting of SEQ ID NO: 1 amino acid positions 89, 90, 95, 0, 33, 34, 35, and 59. The present invention further relates to methods preparing glucose including the use of the polypeptides of the invention for preparing glucose.Type: ApplicationFiled: May 4, 2018Publication date: July 16, 2020Inventors: Birgit Brucher, Andreas Vogel, Hanna Maria Dudek, Rico Czaja
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Publication number: 20200157594Abstract: Methods of preparing highly purified steviol glycosides, particularly rebaudiosides M, D, E and I are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various staring compositions to target steviol glycosides. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.Type: ApplicationFiled: May 15, 2018Publication date: May 21, 2020Inventors: Avetik MARKOSYAN, Siddhartha PURKAYASTHA, Christopher BAYER, Andreas VOGEL, Sabrina KÖPKE, Sebastian BARTSCH, Birgit BRUCHER, Claudia FELLER, Stefan SCHÖNERT, Mathias SALOMO, Thomas SCHULTCHEN
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Publication number: 20200140838Abstract: The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.Type: ApplicationFiled: November 13, 2019Publication date: May 7, 2020Inventors: Stefan SCHOENERT, Mathias SALOMO, Thomas SCHULTCHEN, Andreas VOGEL, Sabrina KOEPKE, Sebastian BARTSCH, Birgit BRUCHER, Claudia FELLER
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Patent number: 10494616Abstract: The invention relates to a cellobiose phosphorylase, which catalyzes, among other things, the synthesis of cellobiose from glucose 1-phosphate and glucose. The cellobiose phosphorylase according to the invention can be understood as a mutation of the cellobiose phosphorylase from Cellulomonas uda. In comparison to cellobiose phosphorylase of the wild type, the cellobiose phosphorylase according to the invention is distinguished by improved activity and process stability, in particular temperature stability, and lower product inhibition and therefore is especially suitable for use in industrial processes.Type: GrantFiled: September 10, 2015Date of Patent: December 3, 2019Assignee: PFEIFER & LANGEN GMBH & CO. KGInventors: Timo Johannes Koch, Thomas Hässler, Birgit Brucher, Andreas Vogel
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Publication number: 20190276808Abstract: The present invention is related to a trehalose phosphorylase comprising an amino acid sequence, wherein the amino acid sequence of the trehalose phosphorylase is at least 80% identical to and/or at least 80% homologous to an amino acid sequence of SEQ ID NO: 1, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are selected from the group consisting of amino acid positions of SEQ ID NO: 1 712, 383, 10, 114, 118, 192, 197, 220, 225, 304, 306, 318, 323, 339, 349, 357, 459, 476, 481, 484, 487, 488, 506, 511, 526, 530, 532, 533, 537, 550, 556, 564, 590, 649, 667, 703 and 705.Type: ApplicationFiled: November 28, 2017Publication date: September 12, 2019Inventors: Birgit Brucher, Andreas Vogel
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Publication number: 20180216086Abstract: The invention relates to a cellobiose phosphorylase, which catalyzes, among other things, the synthesis of cellobiose from glucose 1-phosphate and glucose. The cellobiose phosphorylase according to the invention can be understood as a mutation of the cellobiose phosphorylase from Cellulomonas uda. In comparison to cellobiose phosphorylase of the wild type, the cellobiose phosphorylase according to the invention is distinguished by improved activity and process stability, in particular temperature stability, and lower product inhibition and therefore is especially suitable for use in industrial processes.Type: ApplicationFiled: September 10, 2015Publication date: August 2, 2018Inventors: Timo Johannes Koch, Thomas Hässler, Birgit Brucher, Andreas Vogel