Patents by Inventor Bo-Lennart Johansson
Bo-Lennart Johansson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11439926Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: GrantFiled: December 3, 2019Date of Patent: September 13, 2022Assignee: Cytiva BioProcess R&D ABInventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman, Tobias E. Soderman
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Publication number: 20220266170Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: ApplicationFiled: May 10, 2022Publication date: August 25, 2022Inventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman, Tobias E. Soderman
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Patent number: 10751645Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: GrantFiled: January 29, 2018Date of Patent: August 25, 2020Assignee: GE Healthcare BioProcess R&D ABInventors: Carina Engstrand, Annika Forss, Gunnar Glad, Bo-Lennart Johansson, Hans J Johansson, Jean-Luc Maloisel
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Publication number: 20200101398Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: ApplicationFiled: December 3, 2019Publication date: April 2, 2020Applicant: GE Healthcare BioProcess R&D ABInventors: JAN BERGSTROM, GUNNAR GLAD, BO-LENNART JOHANSSON, JEAN-LUC MALOISEL, NILS NORRMAN, TOBIAS E. SODERMAN
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Patent number: 10562007Abstract: The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.Type: GrantFiled: July 22, 2008Date of Patent: February 18, 2020Assignee: GE Healthcare BioProcess R&D ABInventors: Andreas Axen, Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Nils Norrman
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Patent number: 10493380Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: GrantFiled: June 15, 2016Date of Patent: December 3, 2019Assignee: GE Healthcare BioProcess R&D ABInventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman, Tobias E. Soderman
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Publication number: 20180154281Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: ApplicationFiled: January 29, 2018Publication date: June 7, 2018Applicant: GE Healthcare BioProcess R&D ABInventors: CARINA ENGSTRAND, ANNIKA FORSS, GUNNAR GLAD, BO-LENNART JOHANSSON, HANS J JOHANSSON, JEAN-LUC MALOISEL
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Patent number: 9878266Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: GrantFiled: November 17, 2014Date of Patent: January 30, 2018Assignee: GE Healthcare BioProcess R&D ABInventors: Carina Engstrand, Annika Forss, Gunnar Glad, Bo-Lennart Johansson, Hans J Johansson, Jean-Luc Maloisel
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Publication number: 20170050121Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: ApplicationFiled: June 15, 2016Publication date: February 23, 2017Inventors: JAN BERGSTROM, GUNNAR GLAD, BO-LENNART JOHANSSON, JEAN-LUC MALOISEL, NILS NORRMAN, TOBIAS E. SODERMAN
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Patent number: 9409967Abstract: The present invention is within the field of biomolecule purification. More closely the invention relates to chromatographic purification of insulin using a specific kind of shell beads having an inner core and an outer functionalized layer. The method enables purification at high flow rates and high purity, over 90%.Type: GrantFiled: September 28, 2012Date of Patent: August 9, 2016Assignee: GE Healthcare BioProcess R&D ABInventors: Eggert Brekkan, Kjell Eriksson, Bo-Lennart Johansson, Jamil Shanagar
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Patent number: 9278297Abstract: The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.Type: GrantFiled: June 28, 2011Date of Patent: March 8, 2016Assignee: GE Healthcare Bio-Sciences ABInventors: Jan Bergstrom, Bo-Lennart Johansson
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Patent number: 9266041Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: GrantFiled: June 13, 2011Date of Patent: February 23, 2016Assignee: GE Healthcare Bio-Sciences ABInventors: Bo-Lennart Johansson, Jean-Luc Maloisel, Hans J. Johansson, Asa Ek, Maria Holm, Jafar Saidzadeh
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Patent number: 9259729Abstract: The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalization as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D50V for large particles/D50V for small particles]>1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beads/Total volume beads] is in the range 0.05-0.9.Type: GrantFiled: April 20, 2011Date of Patent: February 16, 2016Assignee: GE Healthcare Bio-Sciences ABInventors: Jan Bergstrom, Bo-Lennart Johansson
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Patent number: 9155980Abstract: The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.Type: GrantFiled: February 20, 2013Date of Patent: October 13, 2015Assignee: GE Healthcare Bio-Sciences ABInventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman
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Patent number: 9044740Abstract: The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention.Type: GrantFiled: March 20, 2008Date of Patent: June 2, 2015Assignee: GE Healthcare Bio-Sciences ABInventors: Jean-Luc Maloisel, Bo-Lennart Johansson
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Publication number: 20150073128Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: ApplicationFiled: November 17, 2014Publication date: March 12, 2015Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: CARINA ENGSTRAND, ANNIKA FORSS, GUNNAR GLAD, BO-LENNART JOHANSSON, HANS J JOHANSSON, JEAN-LUC MALOISEL
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Patent number: 8895710Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: GrantFiled: January 4, 2011Date of Patent: November 25, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Carina Engstrand, Annika Forss, Gunnar Glad, Bo-Lennart Johansson, Hans J. Johansson, Jean-Luc Maloisel
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Publication number: 20140243498Abstract: The present invention is within the field of biomolecule purification. More closely the invention relates to chromatographic purification of insulin using a specific kind of shell beads having an inner core and an outer functionalized layer. The method enables purification at high flow rates and high purity, over 90%.Type: ApplicationFiled: September 28, 2012Publication date: August 28, 2014Inventors: Eggert Brekkan, Kjell Eriksson, Bo-Lennart Johansson, Jamil Shanagar
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Patent number: 8685248Abstract: The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.Type: GrantFiled: February 22, 2010Date of Patent: April 1, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman
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Publication number: 20140017813Abstract: The present invention relates to a method for depletion of undesired molecules and/or enrichment of desired molecules from a sample comprising high abundant as well as low abundant molecules, comprising the following steps: a) providing a separation material comprising a solid phase (beads) comprising an inner porous core material comprising magnetic particles and an outer porous shell with a porosity equal or denser than that of the shell; b) adding the sample to the separation material; c) adsorbing a first fraction of molecules with a molecular weight of 500-50 000 Da in the core and simultaneously excluding a second fraction of molecules from binding to the core and the shell, wherein the molecular weight of the second fraction molecules is at least 5 preferably 10 times higher than the molecular weight of the first fraction and d) eluting the desired molecules from the separation material, wherein step d) and optionally step c) is performed using an oscillating power/field applied over the separation matType: ApplicationFiled: March 29, 2012Publication date: January 16, 2014Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel