Abstract: Provided herein, among other things, is a method for deaminating a double-stranded nucleic acid. In some embodiments, the method may comprise contacting a double-stranded DNA substrate that comprises cytosines and a double-stranded DNA deaminase having an amino acid sequence that is at least 80% identical to any of SEQ ID NOS: 21, 40, 47, 49, 50, 55, 58, 59, 62, 63, 65, 67, 70, 71, 76, 106, 107, 110, 112, 114, 117, 163 and/or 164 to produce a deamination product that comprises deaminated cytosines. Enzymes and kits for performing the method are also provided.

Abstract: Providing herein, among other things, are kits, compositions and methods that relate to DNA fragmentation. An embodiment of a composition provides combining: one or more enzymes capable of nick translating activity, a dNTP mix comprising at least one dNTP having a modified base, and at least one modification-sensitive nicking endonuclease that is prevented from nicking DNA if its recognition site contains the modified base. When the composition is added to a sample comprising a double-stranded DNA template that comprises recognition sites for the modification-sensitive nicking endonuclease, a reaction mix was produced which could be incubated for any time period in excess of about 5 minutes to produce fragments of a desired size of the double-stranded DNA template. In this method, the fragments produced include the modified base and, as such, are not re-nicked by the nicking endonuclease.

Abstract: A vial adapter configured to allow direct fluid withdrawal with a syringe from a fluid container having a self-healing sealing member is described. The syringe is connected to the vial adapter by a vial adapter connecting member and a corresponding syringe connecting member. In use, the fluid container is pressed against the vial adapter, and the vial adapter removably locks the fluid container to the vial adapter. When removably locked, a stake of the vial adapter pierces the sealing member.

Abstract: Various embodiments include an encoder for a motor. The encoder may include: a sensor module for collecting sensor data of the motor with a sensor while the motor is running; a storage module storing motor running records; and a microcontroller unit. The microcontroller is programmed to determine a running state of the motor using the sensor data, storing information about the running state into the storage module as a motor running record in response to a determination that the running state is a predefined running state, and providing at least one of the motor running records stored in the storage module via a communication interface in response to a reading request received from the communication interface.

Abstract: A massage device includes a shell defining a moving space therein, a driving member arranged in the shell, and a massage member connected to the driving member in a transmission way and extending into the moving space. The moving space communicates with the outside environment, and at least a portion of the massage member is capable of extending beyond the moving space of the shell to the outside environment under the driving of the driving member.

Abstract: An insert for a catheter system may include a distal end, a proximal end, and a middle portion disposed between the distal end and the proximal end. The distal end of the insert may include a male port, and the proximal end of the insert may include a female port opposite the male port. The insert may be configured to extend through an adapter and/or a needleless connector of a catheter system to reduce exposure of the adapter and/or the needleless connector to blood, which may flow through a lumen of the insert. The lumen of the insert may extend through the male port, the middle portion, and the female port.

Abstract: An electrical connection socket includes an insulated substrate, and the insulated substrate has an upper surface, a lower surface, and a side surface. The insulated substrate includes at least one plug portion. At least two power terminals are disposed in the electric connection area. The signal connection area is provided with an electric signal slot, an opening of the electric signal slot is located on the side surface of the insulated substrate. Along a direction perpendicular to the lower surface, there is a height difference between the electrical signal slot and the connection section. The electrical connection socket can increase a distance between the power terminal and the electrical signal slot in the electrical connection socket, and increase a distance between the power terminal and a metal housing of a module in a matching direction when the electrical connection socket is matched with the module.

Abstract: A refrigerator appliance or ice supply, as provided herein, may include an ice maker, an ice bucket, and a shutter. The ice maker may include a mold body defining a discrete first compartment and second compartment within which water freezes. The ice bucket may define a first chamber and a second chamber. The second chamber may be separated from the first chamber. The ice bucket may further define an outlet opening having a first portion in fluid communication with the first chamber and a second portion in fluid communication with the second chamber. The shutter may be disposed at the outlet opening and movable between a first position and a second position. The first position may include the shutter covering the second portion and spaced apart from the first portion. The second position may include the shutter covering the first portion and spaced apart from the second portion.

Abstract: A cable trench inspection robot. The cable trench inspection robot includes a base spreading system, and a camera lifting system disposed on the base spreading system, where the base spreading system includes a base plate, a linear action unit, a cantilever plate, a walking assembly, and a jacking assembly, and the camera lifting system includes a travel block, an imitated four-bar assembly, a support rod, and a camera assembly. Such inspection can realize a through-wall operation by using a cable trench, thereby avoiding many use problems caused by a firewall, and efficiently completing a predetermined inspection task.

Abstract: A vascular access system which may be configured for blood draw may include a housing, which may include a distal end, a proximal end, and a slot disposed between the distal end and the proximal end. The slot may include a notch. The vascular access system may include a cannula hub, which may be disposed within the housing and movable with respect to the slot. The cannula hub may include a tab extending through the slot. The vascular access system may include a cannula extending distally from the cannula hub. In response to the tab being disposed within the notch, a distal tip of the cannula may be disposed within the housing. In response to advancing the tab along the slot to a distal end of the slot, the distal tip of the cannula is disposed distal to the distal end of the housing.

Abstract: An example intent-recognition system comprises a processor and memory storing instructions. The instructions cause the processor to receive speech input comprising spoken words. The instructions cause the processor to generate text results based on the speech input and generate acoustic feature annotations based on the speech input. The instructions also cause the processor to apply an intent model to the text result and the acoustic feature annotations to recognize an intent based on the speech input. An example system for adapting an emotional text-to-speech model comprises a processor and memory. The memory stores instructions that cause the processor to receive training examples comprising speech input and receive labelling data comprising emotion information associated with the speech input. The instructions also cause the processor to extract audio signal vectors from the training examples and generate an emotion-adapted voice font model based on the audio signal vectors and the labelling data.

Abstract: An intravenous (IV) catheter assembly may include a catheter adapter and a catheter extending from the distal end of the catheter adapter. The intravenous (IV) catheter assembly may include an introducer needle. The elongated shaft may include a flashback notch, and the flashback notch may include a distal end and a proximal end. The flashback notch may include a smooth tapered edge extending from the distal end in a direction that increases a depth of the flashback notch. The smooth tapered edge may extend along at least half of an entire length of the flashback notch between the distal end and the proximal end.

Abstract: A method for manufacturing a clad steel pipe is provided, wherein the clad steel pipe is manufactured by using a clad steel plate as a raw material. The clad steel plate comprises a base layer (1) and a clad layer (2) roll-bonded with the base layer (1). The method for manufacturing a clad steel pipe comprises the steps of forming, welding, and deburring; both sides of the clad steel plate are bent towards the base layer (1) side of the clad steel plate, then the forming step is carried out, and after the forming step, the opening faces of the resultant pipe blank are all in a form of the clad layer (2). According to the method for manufacturing a clad steel pipe, a clad steel pipe is manufactured by using a clad steel plate as a raw material. Thus, continuance and high efficiency of a high-frequency longitudinal welding pipe unit is fully utilized, subsequent non-continuous processes are not necessary, and the corrosion resistance at the weld of the clad steel pipe is ensured.

Abstract: An ice bucket for a refrigerator appliance, includes a bucket body; an auger provided inside the bucket and including an auger shaft rotatable about a first shaft axis and a collet at a first end of the auger shaft and including an axially beveled surface formed circumferentially around the auger shaft; and an agitator provided in the bucket in mechanical communication with the auger, the agitator including an agitator shaft rotatable about a second shaft axis non-parallel to the first shaft axis, a plurality of first tines extending radially from the agitator shaft within the bucket body to engage ice therein, and a projection that extends perpendicularly from the agitator shaft opposite the plurality of tines. The projection engages with the axially beveled surface to oscillate the agitator.

Abstract: A soil completely-covered structure includes a base layer, slope body filling soil and a buffering layer; the base layer is arranged around a vertical tank body and is made of a base material located on a ground floor, and a first filling gap is formed between the base layer and an outer wall of the vertical tank body; the slope body filling soil is arranged on the base layer, a second filling gap is formed between the side of the slope body filling soil and the outer wall of the vertical tank body, and a side slope surface is formed on the outer side of the slope body filling soil; and the buffering layer is formed in the first filling gap and the second filling gap, and the buffering material fills the first filling gap and the second filling gap to wrap the surface of the vertical tank body.