Abstract: This disclosure provides a method for improving the efficiency and timing of detecting whether sepsis-related microorganisms are present in a fluid sample. The method comprises the steps of: collecting the fluid sample from a patient; fractioning the fluid sample to isolate a quantity of microorganism cells; extracting a portion of the microorganism cells from the fluid sample; lysing a portion of the microorganism cells extracted from the fluid sample to extract microorganism DNA; amplifying the microorganism DNA from the microorganism cells from a predetermined set of DNA primers to determine whether sepsis-related microorganisms are present within the fluid sample.

Abstract: This disclosure provides a method for improving the efficiency and timing of detecting whether sepsis-related microorganisms are present in a fluid sample. The method comprises the steps of: collecting the fluid sample from a patient; fractioning the fluid sample to isolate a quantity of microorganism cells; extracting a portion of the microorganism cells from the fluid sample; lysing a portion of the microorganism cells extracted from the fluid sample to extract microorganism DNA; amplifying the microorganism DNA from the microorganism cells from a predetermined set of DNA primers to determine whether sepsis-related microorganisms are present within the fluid sample.

Abstract: This disclosure provides a method for detecting bacterial and fungal pathogens at clinically relevant concentrations in a clinical fluid sample using a visual color change test. The method includes the steps of: preparing the clinical fluid sample for heat lysing with water; heat lysing the clinical fluid sample to form a lysate that includes DNA and RNA forming a first mixture; mixing the lysate with a target primer set, EBT dye, a polymerase enzyme, and a chemical reaction buffer in a vial to form a reaction mixture; incubating the reaction mixture; amplifying the optional DNA and RNA and the target primer in the reaction mixture using LAMP; cooling the reaction mixture for a predetermined amount of time stopping the amplification; and identifying a color change in the reaction mixture that is indicative of the presence of bacterial or fungal pathogens.