Abstract: Provided herein are delivery particle systems (XDP) useful for the delivery of payloads of any type. In some embodiments, a XDP particle system with tropism for target cells of interest is used to deliver CRISPR/Cas polypeptides (e.g., CasX proteins) and guide nucleic acids (gNA), for the modification of nucleic acids in target cells. Also provided are methods of making and using such XDP to modify the nucleic acids in such cells.

Abstract: Provided herein are Class 2 Type V CRISPR systems comprising CRISPR-Cas polypeptides, (e.g., CasX:gRNA systems comprising CasX polypeptides), guide nucleic acids (gRNA), and optionally donor template nucleic acids, useful in the modification of a PTBP1 gene. The systems are also useful in methods for reprogramming certain eukaryotic cells into functional neurons by the knocking down or knocking out the PTBP1 gene in those cells. Also provided are methods of using such systems in methods of treatment of a subject with a PTBP1-related disease.

Abstract: Provided herein are CasX:gNA systems comprising CasX polypeptides, guide nucleic acids (gNA), and optionally donor template nucleic acids useful in the modification of a SOD1 gene. The systems are also useful for introduction into cells, for example eukaryotic cells having mutations in the SOD1 protein or the SOD1 regulatory element. Also provided are methods of using such CasX:gNA systems to modify cells having such mutations and utility in methods of treatment of a subject with a SOD1-related disease.

Abstract: A method for encoding domain name information into flow records includes receiving a flow record. The flow record includes initial network flow information in a standard flow record format including at least a source address and a destination address. Domain name information associated with each of the source address and destination address is retrieved from a database. The domain name information is encoded into the received flow record while maintaining the initial network flow information to yield an enhanced flow record.

Abstract: A method for encoding domain name information into flow records includes receiving a flow record. The flow record includes initial network flow information in a standard flow record format including at least a source address and a destination address. Domain name information associated with each of the source address and destination address is retrieved from a database. The domain name information is encoded into the received flow record while maintaining the initial network flow information to yield an enhanced flow record.

Abstract: A collapsible aquatic enclosure apparatus is described for use in capturing, isolating and removing contaminants and otherwise easily dispersed aquatic sediments and detritus in water bodies. The collapsible aquatic enclosure can also be used for removal and control of targeted aquatic fauna and flora. Once deployed, the collapsible enclosure creates an isolated volume of water above the target aquatic area. Internal plunger and pipe intake structure within the enclosure provide agitation to remove contaminants, sediments and detritus and targeted fauna and flora residues inside the collapsible apparatus. Methods for using the collapsible aquatic enclosure apparatus are also described.

Abstract: The primary function of the collapsible enclosure is to capture, isolate, and allow for the permanent removal of otherwise easily dispersed contaminates in aquatic sediments and detritus of water bodies. Once deployed, the collapsible enclosure creates an isolated volume of water above these media. Contaminated sediments and detritus covered by the enclosure can then be efficiently removed either by their dissociation from sediment and detritus and into a soluble form within the enclosed water volume or by suspension of contaminated sediments and detritus within the enclosure. The dissolved or suspended contaminants within the enclosure could then be removed by an external pump, for treatment or disposal of recovered water and solids. This invention can also control invasive, noxious or nuisance densities of aquatic plants and animals (such as zebra mussels) by placing enclosures over the area of concern and creating appropriate physical or chemical conditions within the enclosure.