Abstract: The present invention discloses a modified tumor necrosis factor-alpha converting enzyme (TACE) catalytic domain, that unlike the native TACE catalytic domain, is stable at high protein concentrations. The present invention further discloses methods for generating crystals of the modified TACE protein in protein-ligand complexes with a number of inhibitors. In addition, the present invention discloses methods of using the proteins, crystals and/or three-dimensional structures obtained to identify compounds that can modulate the enzymatic activity of TACE.

Abstract: The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of ?-secretase (BACE) polypeptide. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.

Abstract: A lift having a safety lock system includes a mast that may be supported by a base, and a frame that can be lifted or lowered along the mast. The safety lock system includes a first support beam that is located adjacent to the mast and a second support beam that is located opposite the first support beam relative to the mast. The safety lock system also includes a first lock and a second lock. Each of the first lock and the second lock are operable between a plurality of locked positions and an unlocked position. In the locked position, the first lock is engaged with the first support beam to lock the lift frame to the first support beam, and the second lock is engaged with the second support beam to lock the lift frame to the second support beam. Each of the first lock and the second lock can be placed in the unlocked position, wherein the lift frame can be freely lifted or lowered relative to the mast.

Abstract: A lift having a safety lock system includes a mast that may be supported by a base, and a frame that can be lifted or lowered along the mast. The safety lock system includes a first support beam that is located adjacent to the mast and a second support beam that is located opposite the first support beam relative to the mast. The safety lock system also includes a first lock and a second lock. Each of the first lock and the second lock are operable between a plurality of locked positions and an unlocked position. In the locked position, the first lock is engaged with the first support beam to lock the lift frame to the first support beam, and the second lock is engaged with the second support beam to lock the lift frame to the second support beam. Each of the first lock and the second lock can be placed in the unlocked position, wherein the lift frame can be freely lifted or lowered relative to the mast.